Figure 5

Cell cycle analysis of extracts of A. muricata on HL-60 cells. After 24 h, treated cells were incubated with RNAse (40 μg/mL), stained with propidium iodide (25 μg/mL), and analyzed by BD-FACS Caliber flow-cytometer; (A): Leaf; (B): Roots; (C): twigs; Cells distribution after treatment with extracts of A. muricata; values are expressed as means ± standard error (n = 3). Values affected with different letters are significantly different (p < 0.05) from the control. L: Leaf; R: Roots; T: twigs; Ctl: control; app: apoptic phase.