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Table 1 Primer sequences used in RT-PCR analysis

From: Cytotoxicity effect of degraded and undegraded kappa and iota carrageenan in human intestine and liver cell lines

Gene

Primer

Sequences

Expected size (bp)

Annealing temp. (°C)/time (s)

# of cycles

GAPDH

Forward

5′ CCATGGAGAAGGCTGGG 3′

195

55°C/30s

30

Reverse

5′ CAAAGTTGTCATGGATGACC 3′

PCNA

Forward

5′ TCCCACGTCTCTTTGGTGC 3′

155

60°C/30s

30

Reverse

5′ TCTTCGGCCCTTAGTGTAATGAT 3′

MKI67

Forward

5′ GGAAAGTAGGTGTGAAAGAAGAGG 3′

458

50°C/60s

30

Reverse

5′ GCCTTTATCCTCATCTCCTGGTAC 3′

Survivin

Forward

5′ GGCATGGGTGCCCCGACGTT 3′

439

62°C/60s

30

Reverse

5′ AGAGGCCTCAATCCATGGCA 3′