Gene | Primer | Sequences | Expected size (bp) | Annealing temp. (°C)/time (s) | # of cycles |
---|---|---|---|---|---|
GAPDH | Forward | 5′ CCATGGAGAAGGCTGGG 3′ | 195 | 55°C/30s | 30 |
Reverse | 5′ CAAAGTTGTCATGGATGACC 3′ | ||||
PCNA | Forward | 5′ TCCCACGTCTCTTTGGTGC 3′ | 155 | 60°C/30s | 30 |
Reverse | 5′ TCTTCGGCCCTTAGTGTAATGAT 3′ | ||||
MKI67 | Forward | 5′ GGAAAGTAGGTGTGAAAGAAGAGG 3′ | 458 | 50°C/60s | 30 |
Reverse | 5′ GCCTTTATCCTCATCTCCTGGTAC 3′ | ||||
Survivin | Forward | 5′ GGCATGGGTGCCCCGACGTT 3′ | 439 | 62°C/60s | 30 |
Reverse | 5′ AGAGGCCTCAATCCATGGCA 3′ |