Concentration and time effects of degraded and undegraded carrageenan in Fa2N-4 cell line, as measured by the MTT assay. Fa2N4 cells were subjected to the treatment of (A) FGKC, (B) DKC (C) CGKC (D) FGIC and (E) CGIC at concentrations of 62.5 – 2000.0 μg mL−1 and (F) tamoxifen at concentrations of 0.625- 20.000 μg mL−1 for 24 – 72 h. All of the degraded carrageenan showing IC50 in Fa2N-4 cells (black bold line). Data are reported as means of three replicates from 3 independent experiments (n = 3). α: significant difference at 24 h; β: significant difference at 48 h and *: significant difference at 72 h for degraded carrageenan. Significant differences of undegraded carrageenan were in red symbols.