Concentration and time effects of degraded and undegraded carrageenan in HepG2 cell line, as measured by the MTT assay. HepG2 cells were subjected to the treatment of (A) FGKC, (B) DKC (C) CGKC (D) FGIC and (E) CGIC at concentrations of 62.5 – 2000.0 μg mL−1 and (F) tamoxifen at concentrations of 0.625- 20.000 μg mL−1 for 24 – 72 h. Degraded FGKC, DKC and CGKC also showed IC50 in HepG2 cells (black bold line). FGIC had IC50 > 1000 μg mL−1on the cells. Data are reported as means of three replicates from 3 independent experiments (n = 3). α: significant difference at 24 h; β: significant difference at 48 h and *: significant difference at 72 h for degraded carrageenan. Significant differences of undegraded carrageenan were in red symbols.