Figure 4From: The number of intestinal bacteria is not critical for the enhancement of antitumor activity and reduction of intestinal toxicity of irinotecan by the Chinese herbal medicine PHY906 (KD018) Effect of PHY906 on CPT-11-induced inflammation in murine Colon-38 tumor-bearing BDF1 mice with or without (w/o) antibiotic treatment on day 4. (A) Neutrophil infiltration in the middle jejunum section after different treatments. Photographs were taken at 200x magnification. (B and C) qPCR for TNFα and MCP-1 in the middle jejunum after treatment. β-actin was used as an internal control. Each spot represents the mean from two or three different experiments (triplicate samples of each; N = 5). (D-F) Detection of TNF-α, G-CSF and IL6 protein in the plasma after different treatments. (Each spot represents the mean from triplicate samples of each plasma sample; N = 5). Student's t-test was used to determine whether differences between treatment groups were significant. Details of experimental procedures are given in Methods.Back to article page