Day of Experiment | Methodology |
---|---|
Day 1 | 1° injection of prednisolone |
Day 2 | Inoculation of C. albicans in the oral cavity of mice |
Day 5 | 2° injection of prednisolone |
Day 6 | Treatment of oral candidiasis with M. alternifolia essential oil or physiological solution (control group) |
Recovery of C. albicans from the tongue dorsum of mice before and immediately after experimental treatment | |
Day 7 | Euthanasia of the mice: macroscopic analysis, optical microscopy, and Scanning Electron Microscopy (SEM) of the tongue dorsum of mice. |