Figure 1From: Huachansu injection inhibits metastasis of pancreatic cancer in mice model of human tumor xenograft Inhibitory effects of HCS on the viability and invasive behavior of pancreatic cancer cells. (A) Effects of HCS on proliferation of pancreatic cancer cell lines (SW1990, BxPC3 and CF-PAC1) after 48 hours. (B) Effects of HCS on proliferation of SW1990 cells after 36, 48 and 72 hours. (C) IC50=0.545mg/ml was calculated in the time-dose-curve at 48 hour for SW1990. (D) After 24 hours of incubation, cells that migrated to the lower side of the chamber were stained with 1% crystal violet and counted under a microscope. (E) The invasive capability of SW1990 cells was reduced in a dose-dependent manner. (F-I) 24 hours after HCS (50μg/ml) treatment, the mRNA expression of MMP-2 and MMP-9 was reduced by 32.8±2.6% and 37.4±2.8% respectively (F,G); the protein expression of decreased by 33.7±2.5% and 43.8±3.7% respectively (H,I), as compared to the Control (P<0.05). Data are from three independent experiments. In the bar graph, *: P<0.05 indicates the difference significant statistically, compared to controls; “ns” presents no significant difference.Back to article page