Effect of AS extract on RANKL-induced MAPKs and NF-κB activation. AS extract inhibits the p38, ERK, JNK, p65 phosphorylation and I-κB degradation in RANKL-stimulated BMMs. BMMs were pretreated with or without AS extract (50 mg/l) for 1 h prior to RANKL stimulation (100 ng/ml) at indicated time periods. Cells were lysed in lysis buffer, and lysates were analyzed by Western blotting with indicated antibodies. The intensities of protein bands were analyzed and normalized to actin. Similar results were obtained in at least 3 ndependent iexperiments.