Table 1 Validation parameters for the HPLC method for quantitative analysis of phenolic compounds in the shoots of R. idaeus
Compound | Calibration curve | r2 | LOQ [μg/ml] | Recovery |
|---|---|---|---|---|
Gallic acid | y = 0.00022x | 0.9998 | 3.6 | 110.13 ± 3.86 |
Chlorogenic acid | y = 0.000265x + 1.666 | 0.99972 | 4.2 | 107.44 ± 5.76 |
Catechin | y = 0.000409x-0. 222 | 0.99955 | 5.7 | 94.83 ± 4.98 |
Epicatechin | y = 0.000331848x - 1.007753 | 0.99995 | 2.6 | 101.25 ± 6.02 |
Isoquercetin | y = 0.000485465x - 0.0266931 | 0.99997 | 1.25 | 103.07 ± 3.98 |
Ellagic acid | y = 0.000300x + 1.909 | 0.99964 | 2.32 | 102.85 ± 2.98 |
Hyperoside | y = 0.000413836x - 0.29958 | 0.99995 | 1.5 | 105.62 ± 2.43 |
Procyanidin B1 | y = 0.000711836x - 2.04033 | 0.99996 | 17.1 | 105.67 ± 4.15 |
Procyanidin B2 | y = 0.000896x - 2.34 | 0.9999 | 12.6 | 96.42 ± 3.58 |
Sanguiin H-6 | y = 0.00109921x + 4.56879 | 0.99997 | 7.1 | 95.82 ± 5.23 |