Figure 2

Acridine orange (AO)–propidium iodide (PI) double staining cell morphological assessment. Morphological changes in SH-SY5Y cells pretreated with Oryzanol-rich fraction (ORF) for 24 h followed by subsequent exposure to 250 μM H₂O₂ for 24 h. (A) untreated cells (control); (B) 250 μM H₂O₂ alone; (C) 100 μ g/mL ORF +250 μM H₂O₂. Viable cells are stained green by acridine orange; Late apoptotic and necrotic cells are stained orange and red by propidium iodide.