Heavy metals analysis, phytochemical, phytotoxic and anthelmintic investigations of crude methanolic extract, subsequent fractions and crude saponins from Polygonum hydropiper L

Table 2 Phytochemical constituents in crude extract of P. hydropiper

S. no	Phytochemical class	Test performed	Observations	Results
1	Alkaloids	Dragendorff’s Test	Turbidity/precipitation	+
2	Flavonoids	Ferric chloride test	Formation of yellow color which changed to colorless on acid addition	+
3	Saponins	Froth Test	1. Stable froth formation	+
3	Saponins	Froth Test	2. Emulsion formation after olive oil	+
4	Tannins	Gelatin Test	appearance of white precipitate	+
5	Steroids	Liebermann Burchard test	green to pink color was absent	-
6	Glycosides	Keller Killiani	Lower reddish brown layer & upper acetic acid layer which turns bluish green	+
7	Anthraquinones	Bontrager’s test	Formation of red, violet or pink color f in aqueous layer	+
8	Triterpenoids	Liebermann Burchard test	Appearance of reddish brown-deep red color	+

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