- Research article
- Open Access
- Open Peer Review
Vitamin E attenuates neurotoxicity induced by deltamethrin in rats
© Galal et al.; licensee BioMed Central Ltd. 2014
- Received: 30 June 2014
- Accepted: 26 November 2014
- Published: 2 December 2014
The safety of Deltamethrin (DM) has been raised as a point of concern. The current investigation was envisaged to explore the responsiveness of oxidative stress parameters, DNA fragmentation and expression levels of TP53, cycloxygenase 2 (COX2) and cytochrome p4502E1 (CYP2E1) as toxicological endpoint in rats treated with DM. as well as attention was provided to the neuroprotective effect of vitamin E (VE).
Four different groups of rats were used in this study, group I served as control, group II received DM (0.6 mg/kg BW), group III received both DM plus VE and finally group IV received VE only (200 mg/kg BW). The treatment regimen was extending for one month for all groups and the brain tissues were collected for further analysis.
The obtained results showed a highly statistically significant increase in lipid peroxidation (LPO) content, nitric oxide concentration, and DNA fragmentation percentage and expression level of CYP2E1, TP53 and COX2 genes, in addition statistical significant reduction in total antioxidant capacity in DM treated group as compared to control were detected. Oral administration of VE attenuated the neurotoxic effects of DM through improvement of oxidative status, DNA fragmentation percentage and suppressing the expression level of CYP2E1, TP53 and COX2 genes.
From this study we concluded that VE supplementation has beneficial impacts on DM neurotoxicity in rats through its antioxidant and antiapoptotic properties.
- Oxidative stress
- Vitamin E
Although pesticides become instrumental in achieving a significant elevation in crop productivity, they cause serious ecological hazards to the non-target organism . According to WHO  roughly three million cases of pesticide poisonings occur annually and an excess of 250,000 deaths worldwide had been reported. The human exposures to pyrethroids were increased since they had been replaced the organophosphorus insecticides . Owing to its low toxicity and its high potency in eradication, DM had become an insecticide of choice in most countries . Although initially thought to be the safest available insecticide, a number of recent reports have been published on its toxicity effect on human, domestics and experimental animals [5, 6]. The direct exposure to DM vapours or consumption of polluted food and water are the most common routes of intoxication . DM induced several pathological changes including inhibition of mitotic index, chromosomal aberrations , and induction of histological alterations in several important organs . Recently, attention has been focused on the potential relationship between DM exposure and neurodegenerative diseases [10, 11]. DM creates serious problem because its accumulation in fatty tissue like brain . Whereas the exact mechanism of DM induced neurotoxicity is still poorly understood. Accumulation of DM in body systems increases the reactive oxygen species (ROS) production leading to oxidative stress and apoptotic cell death . The deleterious effects of free radicals accumulation included damage to all macromolecules including proteins, lipids, and nucleic acids. This is believed to be involved in the etiology of many neurodegenerative diseases [12, 13]. Under normal circumstances, the body is endowed with effective antioxidant systems to combat the menace of oxidative stress. Though, in extreme oxidative challenge, such as those observed in pesticide poisoning , the body’s antioxidants machineries are overwhelmed. Vitamin E is considered as the most important lipid-soluble antioxidant that protects the brain from oxidative hazard. Many authors reported the neuroprotective influence of VE [15, 16]. VE acts upon cell membranes and has the ability to neutralize compounds which may potentially disrupt membrane stability . Administration of VE decreasing the rate of LPO , decreases the autophagy and neuronal death , prevents the cytochrome oxidase dysfunction, decreases high energy phosphate compounds and NO in different brain regions as well as it increases the expression level of antiapoptotic gene Bcl-2 leading to decreased severity of neuronal damage. Although previous studies have already presented the toxicity induced by DM, data on neurodegenerative toxicity are scarce. Moreover, there are very limited studies evaluating the neuroprotective effect of VE against DM toxicity. Therefore; the current study was carried out to investigate the protective influence of VE against DM neurotoxicity through monitoring its effect on oxidative status, DNA fragmentation percentage and the expression level of CYP2E1, TP53and COX2 genes in brain tissue.
Forty male albino rats, weighing 150–170 g were maintained under standard conditions with free access to food and water. The animals were reared according to the principles of the “Guide for the care and Use of Laboratory Animals” prepared by Beni-Suef University. The Animal care and Use committee of Beni-Suef University approved the study. All efforts were made to minimize animal suffering.
Deltamethrin (>99% pure) was obtained from KZ pesticide company (Egypt). Vitamin E (α tocopherol) and the rest of chemicals were purchased from Sigma Aldrich chemicals, USA.
After two weeks of acclimation, rats were randomly divided into four equal groups. The group (I) administered corn oil (1 ml/kg BW) and act as control group. The group (II) received oral dose of DM (0.6 mg/kg BW). The group (III) was orally received DM (0.6 mg/kg BW) in combination with VE at a dose of (200 mg/kg BW). The group (IV) was given VE only (200 mg/kg BW). All treatments were continued for 30 days and taken once daily via oral gavage. DM and VE were prepared by dissolving in corn oil. The selected dose of DM was based on previous studies in which 1/10 LD50 induced biochemical alteration in rat without morbidity . Animals were treated with VE in dose that was proved to have neuroprotective effect .
All the animals were sacrificed at the end of experiment. Brain was immediately removed and used for further biochemical analysis, DNA fragmentation assay and RNA extraction.
Brain samples were homogenized in 0.1 M cold phosphate buffer saline (pH 7.4) using Teflon pestle. The homogenates were centrifuged at 14,000 × g for 15 min at 4°C. The supernatant was used for measurement of neural LPO expressed as malondialdehyde (MDA) content according to method described by Placer et al. , nitric oxide concentration (NO)  and total antioxidant capacity (TAC) using commercial kit (purchased from Bio diagnostic company, Egypt).
DNA fragmentation assay
Apoptotic changes in the brain tissue were evaluated colorimetrically by DNA fragmentation percentage using the diphenylamine (DPA) assay and DNA laddering assay using agarose gel electrophoresis according to the method described by Perandones et al. . Brain samples were homogenized in hypotonic lysis buffer and centrifuged for 15 min at 14,000 rpm. The supernatants containing small DNA fragments were separated; one-half of the volume was used for gel electrophoresis and the other half together with the pellet containing large pieces of DNA were used for quantification of fragmented DNA.
Isolation of total RNA and real-time PCR (qPCR)
TP53 Exon 7
GTG GTA CCG TAT GAG CCA CC
CAA CCT GGC ACA CAG CTT CC
AAA GCC TCGTCCAGATGCTA
The data were statistically analyzed by SPSS version 16.0 statistical package. Data expressed as the mean ± SE. Differences between the groups were assessed using one way analysis of variance (ANOVA). Differences were considered statistically significant at P < 0.05.
Oxidative stress parameters
Effect of DM treatment on oxidative stress parameters and protective influence of VE supplementation
MDA (U/g tissue)
3.17 ± 0.25b
10.99 ± 1.16a
5.75 ± 0.98ab (47.6%)
2.93 ± 0.84b
2.6 ± 0.4b
6.98 ± 0.25a
4.1 ± 0.12ab (41.24%)
2.5 ± 0.33b
TAC (μ mole/L)
0.289 ± 0.003b
0.15 ± 0.0057a
0.25 ± 0.007ab (66.6%)
0.29 ± 0.009b
DNA fragmentation assay
In the present investigation we employed advanced methods to uncover the molecular mechanisms of neurotoxicity and apoptotic effect of DM in rat's brain. We assessed the effect of DM on oxidative status, DNA fragmentation percentage and the gene expression level of TP53, COX2 and CYP2E1 and also evaluated the ameliorative role of VE; hence the neurodegenerative studies of DM are limited. ROS are continuously produced inside the mammalian body . Oxidative stress occurred as a consequence of imbalance between the production of free radicals and the antioxidative process leading to instabilities in cellular physiology and apoptotic cell death . In general, pesticides intoxication produces oxidative stress through over production of free radicals and induces tissue LPO in mammals and other organisms . The reduced concentration of TAC and elevation of MDA and NO concentration observed in our present study, suggested that DM causes neuronal damage and the pathogenesis may be through the generation of free radicals and oxidative hazard which certainly play a vital role in the pathogenesis of brain injury. Those results are consistent with the literature [17, 31–34]. The brain tissue contains large amounts of oxidizable substrates such as polyunsaturated fatty acids which are the main target of ROS causing LPO . NO is a potent free radical known to be cytotoxic to neurons and glial cells through its reaction with superoxide and generation of highly reactive radical peroxynitrite . Accumulation of DM in body systems increases the ROS production and leads to depletion of antioxidant parameters which was monitored by significant reduction in TAC (Table 2). The hydrophobic lipophilic nature of DM may be the main causative agent for its accumulation in body systems; ROS production and DNA damage . DNA damage has been proposed as useful parameter for assessing the genotoxic properties of environmental contaminants . DNA fragmentation is considered as a marker and typical characteristic feature of apoptosis . Oral administration of DM had significantly increased DNA damage percentage (Figure 1a). Those results come in the line with data reported by [11, 39]. the oxidative stress induced by DM causes depletion of mitochondrial energy through inhibition of Na, K-ATPases, induction of proteolytic enzymes leading to DNA fragmentation and apoptosis . Also inhibition of ATPases reduces energy supply in neurons leading to neuronal damage . In the same consequence DM caused significant elevation to neutrophiles which play essential role in free radical mediated injury through the extracellular release of superoxide, which is cytotoxic . Our results reported in (Figure 2) are in accordance with other works [5, 43] which suggested that DM induces neurodegeneration and apoptotic cell death in the brain tissue. The ability of DM to cause apoptosis may be contributed to its potential to initiate a series of cell death signaling events that finally lead to DNA fragmentation . According to the results in our study, oral administration of DM increases the mRNA expression of the pre-apoptotic gene TP53 (Figure 2a). Such result is in harmony with those obtained by [38, 43, 44]. Increased ROS production and subsequent DNA damage might be reasons for this overexpression . In oxidative stress p53 protein is activated through multiple posttranslational events including phosphorylation . Activated p53 triggers a number of signaling pathways that may lead to cell cycle arrest and apoptosis . In the CNS, the apoptotic gene (COX2) is expressed under normal conditions . Moreover, it has been demonstrated that COX2 expression is regulated by TP53 gene . According to our finding we detected that, overexpression of COX2 upon DM toxicity. Kim et al.  reported that ROS are considered as mediators for COX2 expression. ROS serve as intracellular signals for activation of gene expression through the involvement of specific redox-sensitive signaling pathways and transcription factors . The pervious findings regarding the metabolism of DM revealed that the vast majority of its metabolism occurs in the liver while the extrahepatic metabolism has been reported in other tissues like brain . In rats, the main reaction involved in DM detoxification is ester cleavage, by cytochrome P450 (CYP) enzymes and carboxyesterase action . CYP2E1 is a widely distributed isoform in the brain and is the main inducible CYP against environmental chemicals . The regulation of CYP2E1 expression was occurred through several mechanisms such as protein stabilization and increased translational efficiency which implicated by xenobiotics . Although CYP2E1 plays important role in neuronal detoxification , its induction in the brain could increase the risk of neurotoxicity. CYP2E1 bioactivities several cytotoxins to their reactive intermediates and generate ROS and substrate-derived radicals especially when it is induced . Those may be the main cause for CYP2E1 overexpression after DM intoxication (Figure 2c). Those results were in the line with that reported by [53, 57]. In the current study, the oral administration of VE (200 mg/kg) as antioxidant proved to reduce the neurotoxicity induced by DM through statistical significant reduction for MDA, NO concentration and elevation of the TAC (Table 2). Moreover, VE significantly reduced the DNA fragmentation percentage (Figure 1a) and DNA laddering pattern (Figure 1b). In addition VE attenuated the overexpression of TP53 by 37.2%, COX2 by 50% and CYP2E1 by 41.8%, in compared to DM treated group (Figure 2). Oxidative stress plays a major role in DM induced toxicity. Vitamin E is a potent antioxidant which is reported to ameliorate the effect of many known chemotherapeutic agents and pesticides as well. Many previous literatures showed the protective effect of VE against DM intoxication [58–60]. Vitamin E plays a central role against oxidative damage in neuronal tissue as it is the major lipid-soluble chain breaking antioxidant which effectively protects against neuronal damage . VE maintained the activities of membrane bound enzymes at near normal values and thus preserving mitochondrial membrane integrity and protected enzyme activities from oxidation by free radicals . The protective effect of VE against DNA damage comes in the line with the result reported by . VE play an important role in prevention of neuronal death not only by elimination of ROS through its antioxidant ability, but also through non-antioxidant properties like influencing cellular signaling and transcriptional control [63, 64].
In the current investigation we can concluded that, DM is a potential neurotoxic pesticide, it induced degeneration and apoptotic cell death in rat's brain. On the other hand, VE is proved to be a potent antioxidant which ameliorates the adverse effects triggered by DM.
- Yonar ME, Sakin F: Ameliorative effect of lycopene on antioxidant status in Cyprinus carpio during pyrethroid deltamethrin exposure. Pest Biochem Physiol. 2011, 99: 226-231. 10.1016/j.pestbp.2010.12.008.View ArticleGoogle Scholar
- WHO: The impact of pesticides on health. 2004, Geneva: World Health OrganizationGoogle Scholar
- Khan DA, Bhatti MM, Khan FA, Naqvi ST, Karam A: Adverse effects of pesticides residues on biochemical markers in Pakistani tobacco farmers. Int J Clin Exp Med. 2008, 1: 274-282.PubMedPubMed CentralGoogle Scholar
- Koprucu SS, Yonar E, Seker E: Effects of deltamethrin on antioxidant status and oxidative stress biomarkers in freshwater mussel, Unio elongatulus eucirrus. Bull Environ Contam Toxicol. 2008, 81: 253-257. 10.1007/s00128-008-9474-x.View ArticlePubMedGoogle Scholar
- Sharma P, Jan M, Singh R: Deltamethrin toxicity. Ind J Biol Stud Res. 2013, 2 (2): 91-107.Google Scholar
- Chandra N, Jain NK, Sondhia S, Srivastava AB: Deltamethrin induced toxicity and ameliorative effect of alpha-tocopherol in broilers. Bull Environ Contam Toxicol. 2013, 90: 673-678. 10.1007/s00128-013-0981-z.View ArticlePubMedGoogle Scholar
- Barlow SM, Sullivan FM, Lines J: Risk assessment of the use of deltamethrin on bednets for the prevention of malaria. Food Chem Toxicol. 2001, 39 (5): 407-422. 10.1016/S0278-6915(00)00152-6.View ArticlePubMedGoogle Scholar
- Agarwal DK, Chauhan LK, Gupta SK, Sundararaman V: Cytogenetic effects of deltamethrin on rat bone marrow. Mutat Res. 1994, 311 (1): 133-138. 10.1016/0027-5107(94)90081-7.View ArticlePubMedGoogle Scholar
- Shona SI, El Aasar EM, Zaki SM, Sayed WM: Morphological and morphometric renal changes in the adult albino rat following oral administration of deltamethrin and the possible protective role of vitamin-E. J Appl Sci Res. 2010, 6 (4): 280-290.Google Scholar
- Tayebati SK, Di Tullio MA, Ricci A, Amenta F: Influence of dermal exposure to the pyrethroid insecticide deltamethrin on rat brain microanatomy and cholinergic/dopaminergic neurochemistry. Brain Res. 2009, 1301: 180-188.View ArticlePubMedGoogle Scholar
- Hossain MM, Richardson JR: Mechanism of pyrethroid pesticide–induced apoptosis: role of Calpain and the ER stress pathway. Toxicol Sci. 2011, 122 (2): 512-525. 10.1093/toxsci/kfr111.View ArticlePubMedPubMed CentralGoogle Scholar
- Rehman H, Ali M, Atif F, Kaur M, Bhatia K, And RS: The modulatory effect of deltamethrin on antioxidants in mice. Clin Chim Acta. 2006, 369: 61-65. 10.1016/j.cca.2006.01.010.View ArticlePubMedGoogle Scholar
- Huang YL, Yu FZ, Si YW, Shi N: NF-E2 related factor 2 activation and heme oxygenase-1 induction by tert-butylhydroquinone protect against deltamethrin- mediated oxidative stress in PC12 cells. Chem Res Toxicol. 2007, 20 (9): 1242-1251. 10.1021/tx700076q.View ArticleGoogle Scholar
- Saxena R, Garg P: Vitamin E provides protection against in vitro oxidative stress due to pesticide (Chlorpyrifos and Endosulfan) in goat RBC. Bull Biosci. 2010, 1: 1-6.Google Scholar
- Gohil K, Vasu VT, Cross CE: Dietary alpha-tocopherol and neuromuscular health: search for optimal dose and molecular mechanisms continues. Mol Nutr Food Res. 2010, 54: 693-709. 10.1002/mnfr.200900575.View ArticlePubMedGoogle Scholar
- Nishida Y, Ito S, Ohtsuki S, Yamamoto N, Takahashi T, Iwata N, Jishage K, Yamada H, Sasaguri H, Yokota S, Piao W, Tomimitsu H, Saido TC, Yanagisawa K, Terasaki T, Mizusawa H, Yokota T: Depletion of vitamin E increases amyloid beta accumulation by decreasing its clearances from brain and blood in a mouse model of Alzheimer disease. J Biol Chem. 2009, 284: 33400-33408. 10.1074/jbc.M109.054056.View ArticlePubMedPubMed CentralGoogle Scholar
- Leonarduzzi G, Sottero B, Poli G: Targeting tissue oxidative damage by means of cell signaling modulators: the antioxidant concept revisited. Pharmacol Therapeut. 2010, 128 (2): 336-374. 10.1016/j.pharmthera.2010.08.003.View ArticleGoogle Scholar
- Cao L, Chen R, Xu J, Lin Y, Wang R, Chi Z: Vitamin E inhibits activated chaperone-mediated autophagy in rats with status epilepticus. Neuroscience. 2009, 161 (1): 73-77. 10.1016/j.neuroscience.2009.02.059.View ArticlePubMedGoogle Scholar
- Numakawa Y, Numakawa T, Matsumoto T, Yagasak IY, Kumamaru E, Kunugi H, Taguchi T, Niki E: Vitamin E protected cultured cortical neurons from oxidative stress-induced cell death through the activation of mitogen-activated protein kinase and phosphatidylinositol 3-kinase. J Neurochem. 2006, 97: 1191-1202. 10.1111/j.1471-4159.2006.03827.x.View ArticlePubMedGoogle Scholar
- Oda SS, El-Maddawy ZK: Protective effect of vitamin E and selenium combination on deltamethrin-induced reproductive toxicity in male rats. Exp Toxico Pathol. 2012, 64 (7–8): 813-819.View ArticleGoogle Scholar
- Nagkrishna L, Tamma NK, Tadvi NA, Shareef M: Evaluation of protective effect of vitamin -e on vincristine induced peripheral neuropathy in albino rats. J Evol Med Dent Sci. 2013, 2 (15): 2514-2521. 10.14260/jemds/570.View ArticleGoogle Scholar
- Placer ZA, Crushman L, Johnson BC: Estimation of product of lipid peroxidation (malondialdhyde) in biochemical systems. Anal Biochem. 1966, 16: 359-364. 10.1016/0003-2697(66)90167-9.View ArticlePubMedGoogle Scholar
- Miranda KM, Espey MG, Wink DA: A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide. 2001, 5 (1): 62-71. 10.1006/niox.2000.0319.View ArticlePubMedGoogle Scholar
- Perandones CE, Lllera AV, Peckham D, Stunzl LL, Ashman RF: Regulation of apoptosis in vitro in mature murine spleen T-cell. J Immunol. 1993, 151: 3521-3529.PubMedGoogle Scholar
- Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔ CT method. Methods. 2001, 25: 402-408. 10.1006/meth.2001.1262.View ArticlePubMedGoogle Scholar
- Victor DL, Hailing L, Zhang F, Chau RL, Subbaramaiah K, Andrew JD, Mark JC: Induction of cyclooxygenase-2 by tumor promoters in transformed and cytochrome P450 2E1-expressing hepatocytes. Carcinogenesis. 2002, 23 (1): 73-79. 10.1093/carcin/23.1.73.View ArticleGoogle Scholar
- Gouda E, Elbehairy A, Ghoneim M: Evaluation of antimutagenic efficacy of some natural compounds on cyclophosphamide-induced p53 alterations using PCR-SSCP. Z Naturforsch. 2008, 63c: 857-863.Google Scholar
- Ko JH, Ibrahim M, Park WS, Ko EA, Kim N, Warda M, Lim I, Bang H, Han J: Cloning of large-conductance Ca2+ activated K+ channel α-subunits in mouse cardiomyocytes. Biochem Biophys Res Commun. 2009, 389: 74-79. 10.1016/j.bbrc.2009.08.087.View ArticlePubMedGoogle Scholar
- Sun Y: Free radicals, antioxidant enzymes, and carcinogenesis. Free Radic Biol Med. 1990, 8: 583-599. 10.1016/0891-5849(90)90156-D.View ArticlePubMedGoogle Scholar
- Saxena R, Garg P, Jain DK: In vitro anti-oxidant effect of vitamin E on oxidative stress induced due to pesticides in Rat erythrocytes. Toxicol Int. 2011, 18 (1): 73-78. 10.4103/0971-6580.75871.View ArticlePubMedPubMed CentralGoogle Scholar
- Chargui I, Grissa I, Bensassi F, Hrira MY, Haouem S: Oxidative stress, biochemical and histopathological alterations in the liver and kidney of female rats exposed to low doses of deltamethrin (DM): a molecular assessment. Biomed Environ Sci. 2012, 25: 672-683.PubMedGoogle Scholar
- Li HY, Wu SY, Ma Q, Shi N: The pesticide deltamethrin increases free radical production and pr omotes nuclear translocation of the stress response transcription factor Nrf2 in rat brain. Toxicol Ind Health. 2011, 27: 579-590. 10.1177/0748233710393400.View ArticlePubMedPubMed CentralGoogle Scholar
- Yousef MI, Awad TI, Mohamed EH: Deltamethrin induced oxidative damage and biochemical alterations in rat and its attenuation by Vitamin E. Toxicology. 2006, 227: 240-247. 10.1016/j.tox.2006.08.008.View ArticlePubMedGoogle Scholar
- Abdel-Daim MM, Abuzead SMM, Halawa SM: Protective role of spirulina platensis against acute deltamethrin-induced toxicity in rats. PLoS One. 2013, 8 (9): e72991-10.1371/journal.pone.0072991. doi:10.1371/journal.pone.0072991View ArticlePubMedPubMed CentralGoogle Scholar
- Floyd RA, Carney JM: Free radical damage to protein and DNA: mechanisms involved and relevant observations on brain undergoing oxidative stress. Ann Neurol. 1992, 32: S22-S27. 10.1002/ana.410320706.View ArticlePubMedGoogle Scholar
- Baraona E, Zeballos GA, Shoichet L, Mak KM, Lieber CS: Ethanol consumption increases nitric oxide production in rats, and its peroxynitritemediated toxicity is attenuated by polyenylphosphatidylcholine. Alcohol Clin Exp Res. 2002, 26 (6): 883-889. 10.1111/j.1530-0277.2002.tb02618.x.View ArticlePubMedGoogle Scholar
- Kohn HW: The significance of DNA damage assays in toxicity and carcinogenicity assessment. Ann NY Acad Sci. 1983, 407: 106-118. 10.1111/j.1749-6632.1983.tb47817.x.View ArticlePubMedGoogle Scholar
- Li GY, Xie P, Li HY, Hao L, Xiong Q, Qiu T: Involment of p53, Bax, and Bcl-2 pathway in microcystins-induced apoptosis in rat testis. Environ Toxicol. 2009, 26: 111-117.View ArticleGoogle Scholar
- Ismail MF, Mohamed HM: Deltamethrin-induced genotoxicity and testicular injury in rats: comparison with biopesticide. Food Chem Toxicol. 2012, 50: 3421-3425. 10.1016/j.fct.2012.07.060.View ArticlePubMedGoogle Scholar
- Yu F, Wang Z, Ju B, Wang Y, Wang J, Bai D: Apoptotic effect of organophosphorus insecticide chlorpyrifos on mouse retina in vivo via oxidative stress and protection of combination of vitamins C and E. Exp Toxicol Pathol. 2008, 59: 415-423. 10.1016/j.etp.2007.11.007.View ArticlePubMedGoogle Scholar
- Liu GP, Ma Q, Shi N: Tyrosine hydroxylase as a target for deltamethrin in the nigrostriatal dopaminergic pathway. Biomed Environ Sci. 2006, 19: 27-34.PubMedGoogle Scholar
- Tewari A, Gill JPS: Assessment of hemato-biochemical parameters on exposure to low level of deltamethrin in mouse model. Vet World. 2014, 7 (3): 152-157. 10.14202/vetworld.2014.152-157.View ArticleGoogle Scholar
- Wu A, Ren T, Hu Q, Liu Y: Deltamethrin induces altered expression of P53, Bax and Bcl and Bcl-2 in rat brain. Neurosci Lett. 2000, 284 (1–2): 29-32.View ArticlePubMedGoogle Scholar
- Elwan MA, Richardson JR, Guillot TS, Caudle WM, Miller GW: Pyrethroid pesticide-induced alterations in dopamine transporter function. Toxicol Appl Pharmacol. 2006, 211: 188-197. 10.1016/j.taap.2005.06.003.View ArticlePubMedGoogle Scholar
- Harris SL, Levine AJ: The p53 pathway: positive and negative feedback loops. Oncogene. 2005, 24: 2899-2908. 10.1038/sj.onc.1208615.View ArticlePubMedGoogle Scholar
- Mihara M, Erster S, Zaika A, Petrenko O, Chittenden T, Pancoska P, Moll UM: p53 has a direct apoptogenic role at the mitochondria. Mol Cell. 2003, 11: 577-590. 10.1016/S1097-2765(03)00050-9.View ArticlePubMedGoogle Scholar
- Minghetti L: Cyclooxygenase-2 (COX-2) in inflammatory and degenerative brain diseases. J Neuropathol Exp Neurol. 2004, 63: 901-910.View ArticlePubMedGoogle Scholar
- Sheng GG, Shao J, Sheng H, Hooton EB, Isakson PC, Morrow JD, Coffey RJ, DuBois RN, Beauchamp RDA: Selective cyclooxygenase 2 inhibitor suppresses the growth of H-rastransformed rat intestinal epithelial cells. Gastroenterology. 1997, 113: 1883-1891. 10.1016/S0016-5085(97)70007-6.View ArticlePubMedGoogle Scholar
- Kim EH, Lee MJ, Kim IH, Pyo S, Choi KT, Rhee DK: Anti-apoptotic effects of Red ginseng on oxidative stress induced by hydrogen peroxide in SK-N-SH cells. J Ginseng Res. 2010, 34 (2): 138-144. 10.5142/jgr.2010.34.2.138.View ArticleGoogle Scholar
- Guha M, Bai W, Nadler JL, Natarajan R: Molecular mechanisms of tumor necrosis gene expression in monocytic cells via hyperglycemia-induced oxidant stress-dependent and -independent pathways. J Biol Chem. 2000, 275: 17728-17739. 10.1074/jbc.275.23.17728.View ArticlePubMedGoogle Scholar
- Godin SJ, Crow JA, Scollon EJ, Hughes MF, DeVito MJ, Ross MK: Identification of rat and human cytochrome P450s and a rat serum esterase which metabolize deltamethrin and esfenvalerate. Drug Metab Dispos. 2007, 35: 1664-1671. 10.1124/dmd.107.015388.View ArticlePubMedGoogle Scholar
- Feyereisen R: Insect cytochrome P450. Comprehensive Molecular Insect Science. Edited by: Gilbert LI, Iatrou K, Gill SS. 2005, Oxford: Elsevier, 1-77. 4View ArticleGoogle Scholar
- Vences-Mejia A, Gomez-Garduno J, Caballero-Ortega H, Dorado-Gonzalez V, Nosti-Palacios R, Labra-Ruiz N, Espinosa-Aguirre J: Effect of mosquito mats (pyrethroid-based) vapor inhalation on rat brain cytochrome P450s. Toxicol Mech Meth. 2012, 22 (1): 41-46. 10.3109/15376516.2011.591448.View ArticleGoogle Scholar
- Novak RF, Woodcroft KJ: The alcohol-inducible form of cytochrome P450 (CYP 2E1): role in toxicology and regulation of expression. Arch Pharm Res. 2000, 23 (4): 267-282. 10.1007/BF02975435.View ArticlePubMedGoogle Scholar
- Nissbrandt H, Bergquist F, Jonason J, Engberg G: Inhibition of cytochrome P450 2E1 induces an increase in extracellular dopamine in rat substantia nigra: a new metabolic pathway?. Synapse. 2001, 40: 294-301. 10.1002/syn.1052.View ArticlePubMedGoogle Scholar
- Lieber CS: Cytochrome P450 2E1: its physiological and pathological role. Physiol Rev. 1997, 77: 517-544.PubMedGoogle Scholar
- de Assis HCS, Nicareta L, Salvo LM, Klemz C, Truppel JH, Calegari R: Biochemical biomarkers of exposure to deltamethrin in freshwater fish, ancistrus multispinis. Braz Arch Biol Technol. 2009, 52 (6): 1401-1407. 10.1590/S1516-89132009000600012.View ArticleGoogle Scholar
- Amin K, Hashem K: Deltamethrin-induced oxidative stress and biochemical changes in tissues and blood of catfish (Clarias gariepinus): antioxidant defense and role of alpha-tocopherol. BMC Vet Res. 2012, 8: 45-10.1186/1746-6148-8-45.View ArticlePubMedPubMed CentralGoogle Scholar
- El Maghraby S, Taha HA: Amelioratory effects of vitamin E against biochemical toxicity induced by deltamethrin in male rats. J Arab Soc Med Res. 2012, 7: 92-96.Google Scholar
- Slima BA, Ben Ali M, Barkallah M, Traore A, Boudawara T, Allouche N, Gdoura R: Antioxidant properties of Pelargonium graveolens L’Her essential oil on the reproductive damage induced by deltamethrin in mice as compared to alpha-tocopherol. Lipids Health Dis. 2013, 12: 30-10.1186/1476-511X-12-30.View ArticlePubMedPubMed CentralGoogle Scholar
- Minamiyama Y, Takemura S, Bito Y, Shinkawa H, Tsukioka T, Nakahira A, Suehiro S, Okada S: Supplementation of alpha-tocopherol improves cardiovascular risk factors via the insulin signaling pathway and reduction of mitochondrial reactive oxygen species in type II diabetic rats. Free Radic Res. 2008, 42: 261-271. 10.1080/10715760801898820.View ArticlePubMedGoogle Scholar
- Shirpoor A, Minassian S, Salami S, Khadem-Ansari MH, Ghaderi-Pakdel F, Yeghiazaryan M: Vitamin E protects developing rat hippocampus and cerebellum against ethanol-induced oxidative stress and apoptosis. Food Chem. 2009, 113: 115-120. 10.1016/j.foodchem.2008.07.034.View ArticleGoogle Scholar
- Ferri P, Cecchini T, Ciaroni S, Ambrogini P, Cuppini R, Santi S, Benedetti S: Vitamin E affects cell death in adult rat dentate gyrus. J Neurocytol. 2003, 32: 1155-1164.View ArticlePubMedGoogle Scholar
- Brigelius-Flohe R, Kelly FJ, Salonen JT, Neuzil J, Zingg JM, Azzi A: The European perspective on vitamin E: Current knowledge and future research. Am J Clin Nutr. 2002, 76: 703-716.PubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/14/458/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.