Table 1 PCR conditions for rat antioxidants, cytokines and acute phase proteins genes
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | PCR cycles and conditions |
|---|---|---|---|
GST (575 bp) | GCTGGAGTGGAGTTTGAAGAA | GTCCTGACCACGTCAACATAG | 35 cycles, 55°C 1 min |
GPx (406 bp) | AAGGTGCTGCTCATTGAGAATG | CGTCTGGACCTACCAGGAACTT | 40 cycles 57°C 1 min |
SOD (410 bp) | AGGATTAACTGAAGGCGAGCAT | TCTACAGTTAGCAGGCCAGCAG | 35 cycles, 55°C 1 min |
Catalase (652 bp) | GCGAATGGAGAGGCAGTGTAC | GAGTGACGTTGTCTTCATTAGCACTG | 35 cycles, 55.5°C 1 min |
IL-1β (218 bp) | ATGGCAACCGTACCTGAACCCA | GCTCGAAAATGTCCCAGGAA | 30 cycles, 61°C 1 min |
TNF-α (256 bp) | CCACCACGCTCTTCTGTCTAC | ACCACCAGTTGGTTGTCTTTG | 30 cycles, 58°C 1 min |
IL-8 (460 bp) | CTCCAGCCACACTCCAACAGA | CACCCTAACACAAAACAGAT | 35 cycles, 56°C 1 min |
IL-10 (320bp) | GGAGTGAAGACCAAAGG | TCTCCCAGGGAATTCAAATG | 30 cycles, 57°C 1 min |
α2 –macroglobulin (325 bp) | GCTCCTGTCTGTTTCCTTAGTT | ATTGGCCTTTCGTGGTTTAG | 30 cycles, 56°C 1 min |
α1-glycoprotein (230 bp) | GCTTTCCTCCTGACAACGCTG | GGCTTTTTGTTGTTTGCTTCTATTTC | 30 cycles, 55°C 1 min |
GAPDH (309 bp) | AGATCCACAACGGATACATT | TCCCTCAAGATTGTCAGCAA | 25 cycles, 52°C 1 min |