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Figure 4 | BMC Complementary and Alternative Medicine

Figure 4

From: Attenuation of early liver fibrosis by herbal compound “Diwu Yanggan” through modulating the balance between epithelial-to-mesenchymal transition and mesenchymal-to-epithelial transition

Figure 4

DWYG treatment recovered the expression ratio of TGF-β1 and BMP-7 in the fibrotic livers of rats induced by CCl 4 . (A) The mRNA expression levels of TGF-β1 and BMP-7 in the liver tissues of different treated group rats were detected by quantitative real-time RT-PCR. Delta-delta-CT was calculated as described in Materials and methods, considering GAPDH as internal control and blank control group as reference control. Data represent the means and standard deviation of 3 independent experiments. *p < 0.05 vs. CCl4-induced model group. (B) The protein expression levels of TGF-β1 and BMP-7 in the liver tissues of different treated group rats were analyzed by Western blotting. Anti-β-actin blotting was used as control for equal protein loading. Selected blots from one typical experiment are presented. The bar graph represents the densitometric analysis of the bands in 3 independent experiments. *p < 0.05 vs. blank control group, **p < 0.05 vs. CCl4-induced model group. (C) The mRNA and protein expression ratios of TGF-β1/BMP-7 in the DWYG-treated and CCl4-induced fibrotic liver tissues were statistically analyzed. Data represent the means and standard deviation of 3 independent experiments. *p < 0.05 vs. CCl4-induced model group, #p < 0.05 vs. CCl4-induced model group.

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