Figure 2
DWYG treatment inhibited collagen deposition and tissue damage in the livers of rats induced by CCl 4 . Paraffin-embedded liver sections of different treatment groups were stained by H & E (A) (original magnification, ×200) and Masson’s trichrome (C) (original magnification, ×100) as described in Materials and methods. Representative micrographs are displayed. (B) The serum levels of aspartate transaminase (AST) and alanine transaminase (ALT) in different treatment groups were respectively detected by commercial ELISA kits. Data represent the means and standard deviation of 3 independent experiments. #p < 0.05 vs. blank control group, *p < 0.05 vs. CCl4-induced model group. (D) The relative fibrosis areas were evaluated by morphometric analysis of Masson’s trichrome-stained liver tissue sections from different treatment groups. Data represent the means and standard deviation of 3 independent experiments. #p < 0.05 vs. blank control group, *p < 0.05 vs. CCl4-induced model group. (E) Hepatic hydroxyproline content of different treatment groups was assessed as described in Materials and methods. Data represent the means and standard deviation of 3 independent experiments. #p < 0.05 vs. blank control group, *p < 0.05 vs. CCl4-induced model group.