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Figure 7 | BMC Complementary and Alternative Medicine

Figure 7

From: Growth arrest and apoptosis via caspase activation of dioscoreanone in human non-small-cell lung cancer A549 cells

Figure 7

Relative mRNA levels of Bax and Bcl-2 in DN-treated A549 cells. A549 cells were treated with 30 μM DN for 24, 48 and 72 h. Total RNA was isolated and converted to single-stranded cDNAs, which were then quantified by real-time PCR using TaqMan probe and primer pairs pre-designed for Bax, Bcl-2 and GAPDH as an endogenous control. Relative mRNA levels of Bax, Bcl-2 and Bax/Bcl-2 ratios in treated A549 cells versus untreated cells are expressed as the mean ± SD of at least three independent experiments. *Statistical significance (p <0.05) versus control at equal incubation periods.

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