Figure 5
Suppression of DN-induced apoptosis by the general caspase inhibitor z-VAD-fmk. Representative histograms from one of at least three independent experiments depict cell cycle distributions. Distributions are shown in (A) for untreated A549 cells or in (B) for A549 cells treated with 30 μM DN or pretreated for 1 h with (C) 2.5 μM or (D) 25 μM pancaspase inhibitor z-VAD-fmk prior to the addition of 30 μM DN and further incubation for 72 h. The sub-G1 peaks (blue peaks) on DNA content histograms represent apoptotic cells and the percentages of cells in sub-G1, G1, S and G2/M phases are listed.