Figure 1

The effects of GL on cell viability and apoptosis in human colorectal cancer cells. HCT116 (A), SW480 (B) and LoVo cells (C) were treated with 0, 50, 100 and 200 μg/ml of GL for 24 and 48 h. Cell viability was measured using MTT assay system and expressed as % cell viability compared to the cell without GL treatment. *P < 0.05 compared to cells without GL treatment. (D) HCT116 and SW480 cells were treated with 0, 25, 50 and 100 μg/ml of GL for 24 h. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibodies against PARP. Actin was used as internal control. (E) HCT116 and SW480 cells were treated with 0, 25, 50 or 100 μM of GL for 24 h. the cytosol fraction was extracted from GL-treated cells, and the cell death was measured using the Cell Death Detection ELISA^PLUS Kit, and expressed as absorbance (A₄₀₅-A₄₉₀). *p < 0.05 compared to cells without GL treatment. (F) MCF-7, MDA-MB-231 and HepG-2 cells were treated with 100 μg/ml of GL for 24 h. Cell viability was measured using MTT assay system and expressed as % cell viability compared to the cell without GL treatment. *P < 0.05 compared to cells without GL treatment.