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Table 3 Antioxidant activities of CPE, AA and PBE based on (a) Scavenging effect using DPPH assay (b) Metal reducing ion capability, and (c) Antioxidant activity using β-carotene bleaching assay

From: Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract

Assay Sample Concentration, μg/ml EC 50
  7.8 15.6 31.3 62.5 125 250 500 1000  
(a) DPPH assay (%) AA 45.03 ± 1.74 50.14 ± 3.20 63.05 ± 6.39 66.63 ± 1.75 72.21 ± 2.21 73.45 ± 2.24 76.48 ± 2.58 77.53 ± 2.67 13.25
  PBE 13.70 ± 0.27 23.92 ± 1.04 38.49 ± 2.26 58.89 ± 3.16 67.43 ± 1.71 69.93 ± 1.91 73.54 ± 1.85 74.86 ± 1.42 49.33
  CPE 10.80 ± 0.63 32.10 ± 0.40 58.98 ± 5.82 87.07 ± 3.09 85.01 ± 4.44 86.81 ± 3.47 84.52 ± 3.23 83.28 ± 0.66 26.10
(b) FRAP assay (μM Fe 2+ ) AA 416.24 ± 78.22abαβ 610.43 ± 3.99aγδ 405.47 ± 42.79bαβ 454.19 ± 31.55 336.24 ± 45.33 534.53 ± 29.57 599.83 ± 100.81 754.02 ± 72.49aγε 204.25
  PBE 398.11 ± 15.95 485.06 ± 39.38bβγ 483.67 ± 53.55aβγ 509.22 ± 46.27 551.44 ± 45.71aγδ 435.61 ± 57.16cβγ 793.94 ± 41.84 587.83 ± 4.71 70.76
  CPE 478.12 ± 46.06 567.86 ± 38.07 390.94 ± 15.23 684.27 ± 18.72aδε 526.66 ± 42.06aβγ 620.68 ± 9.07 638.80 ± 52.31 729.57 ± 57.20 97.30
(c) BCB assay (%) BHT 31.01 ± 3.60 46.83 ± 3.18 59.26 ± 1.46 78.70 ± 3.16 91.72 ± 2.51 98.23 ± 6.09 97.17 ± 2.82 99.17 ± 5.48 24.09
  PBE 3.67 ± 0.27 12.88 ± 2.26 23.20 ± 7.65 25.31 ± 6.89 40.71 ± 8.66cγδ 48.32 ± 7.73 49.59 ± 3.51 63.68 ± 13.15 302.71
  CPE 9.65 ± 1.99 5.15 ± 1.10 5.74 ± 1.48 51.47 ± 5.64 70.50 ± 0.43 81.55 ± 1.08 83.44 ± 0.80 88.08 ± 2.73b€ 84.67
  1. a b c dDifferent alphabet in column means significantly different within sample for each assay.
  2. α β γ δ ε € £different symbol means significantly different within concentration for each sample in row for each assay.