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Figure 5 | BMC Complementary and Alternative Medicine

Figure 5

From: Anti-proliferative of physcion 8-O-β-glucopyranoside isolated from Rumex japonicus Houtt. on A549 cell lines via inducing apoptosis and cell cycle arrest

Figure 5

Apoptosis of A549 cell in vitro. In the figures, Ac-DEVD-CHO abbreviated as Ac, 20, 40 and 80 μg/mL of PG abbreviated as PG20, PG40 and PG80, respectively. (A) The cell Apoptosis was determined by flow cytometry. The cells were treated with 20, 40 and 80 μg/mL of PG. 48 hours, and then cells were trypsinized, washed with PBS and stained using an Annexin V/FITC kit. The cell apoptosis was detected with flow cytometry. Fluorescence intensity for annexin V/FITC is plotted on the x-axis, and PI is plotted on the y-axis. (B) The cells were treated with or without Ac-DEVD-CHO for 3 h, then incubation with 20, 40 and 80 μg/mL of PG for 48 h, then MTT assay was carried out. **p < 0.01 vs. medium; # # p < 0.01 vs. PG.

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