Figure 5

Apoptosis of A549 cell in vitro. In the figures, Ac-DEVD-CHO abbreviated as Ac, 20, 40 and 80 μg/mL of PG abbreviated as PG20, PG40 and PG80, respectively. (A) The cell Apoptosis was determined by flow cytometry. The cells were treated with 20, 40 and 80 μg/mL of PG. 48 hours, and then cells were trypsinized, washed with PBS and stained using an Annexin V/FITC kit. The cell apoptosis was detected with flow cytometry. Fluorescence intensity for annexin V/FITC is plotted on the x-axis, and PI is plotted on the y-axis. (B) The cells were treated with or without Ac-DEVD-CHO for 3 h, then incubation with 20, 40 and 80 μg/mL of PG for 48 h, then MTT assay was carried out. **p < 0.01 vs. medium; ^{# #} p < 0.01 vs. PG.