Effects of β-sitosterol derived from Artemisia capillaris on the activated human hepatic stellate cells and dimethylnitrosamine-induced mouse liver fibrosis

Table 1 Real time PCR primer sequence

Gene		Forward primer (5' → 3')	Reverse primer (5' → 3')
Human	GAPDH	CATGGCCTTCCGTGTTCCTA	GCGGCACGTCAGATCCA
	COL1A1	GAGACTGTTCTGTTCCTTGTGTAACTG	CCCGGTGACACATCAAGACA
	ACTA2	TGCCTGATGGGCAAGTGA	CTGGGCAGCGGAAACG
	MMP1	GATCATCGGGACAACTCTCCT	TCCGGGTAGAAGGGATTTGTG
	MMP2	TGAGCTATGGACCTTGGGAGAA	CCATCGGCGTTCCCATAC
	GFAP	CCGCAGCCCTGAAAGAGA	TTGCTGGACGCCATTGC
Mouse	Gapdh	GCCACATCGCTCAGACACC	CCCAATACGACCAAATCCGT
	Col1a1	CGATGGCGTGCTATGCAA	ACTCGCCCTCCCGTTTTT
	Acta2	CCATGTACCCAGGCATTGCT	GGGAGCGAGGGCTGTGAT

Abbreviations: GAPDH human glyceraldehyde 3-phosphate dehydrogenase gene, COL1A1 human collagen type-1 alpha-1 gene, ACTA2 human alpha-smooth muscle actin gene, MMP1 human matrix metalloproteinase-1 gene, MMP2 human matrix metalloproteinase-2 gene, GFAP human glial fibrillary acidic protein gene, Gapdh mouse glyceraldehyde 3-phosphate dehydrogenase gene, Col1a1 mouse collagen type-1 alpha-1 gene, Acta2 mouse alpha-smooth muscle actin gene.

ISSN: 2662-7671