Effects of β -sitosterol on collagen-1 and α -SMA mRNA expression in DMN-induced mouse liver fibrosis. Relatively expressed Col1a1 (A) and Acta2 (B) levels were measured by real-time quantitative PCR. Experiments were carried out in triplicate. Statistical significance determined by one-way ANOVA; values are means ± SEM; ***, p < 0.001 vs TGF-β- treated group. ###, p < 0.001 vs control group.