Figure 2

Inhibition of mutation and induction of cell death in OCs by incubation with herbal extracts. RAW 264.7 cells were seeded and allowed to differentiate into OCs. The cells were then cultured in the absence (Control) or presence of 10 μM AD, or 1, 10 and 100 μg/ml C. atratum, M. azedarach and C. turtschaninovii extracts. After 3 days, cells were stained with crystal violet and TRAP and examined with a microscope at a magnification of 40× and 100×. Bar, 1 mm (40×) and 100 μm (100×).