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Figure 5 | BMC Complementary and Alternative Medicine

Figure 5

From: Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells

Figure 5

Effects of Equisetum arvense on IL-2 biology of primary human lymphocytes. Medium-, CsA or Equisetum-treated activated (PHA-L: 10 μg/mL) lymphocytes were surface-stained with anti-human CD25 mAbs and were analyzed at early (A; 24 h) or at late (B; 48 h) time points. For IL-2 production analysis, cells were treated for 36 h and were restimulated with PMA (50 ng/mL) and ionomycin (500 ng/mL) for additional 6 h followed by surface-staining with anti-human CD8 mAbs and intracellular cytokine assay with PE-conjugated anti-human IFN-γ mAbs or anti-human TNF-α mAbs (C). Cells were analyzed using flow cytometry. Data were expressed and summarized as mean ± SD of five independent experiments and donors. The asterisks (*P < 0.05, **P < 0.01, ***P < 0.001) represent significant differences from activated untreated controls (CTRL =100%).

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