Figure 4

Effects of Equisetum arvense on activation of primary human lymphocytes. Stimulated CD8⁺ T cells were cultured in the presence of medium, CsA (10 μg/mL) or increasing concentrations (0.05-0.8 μg/mL) of the Equisetum extract. Cells were analyzed for CD69 expression using FITC-labeled anti-human mAbs. (A) Representative dot plots are shown and data were summarized from experiments at early (B; 24 h) or late (C; 48 h) time points from five independent experiments and donors. n.d. = no detection. The asterisks (**P < 0.01, ***P < 0.001) represent significant differences from activated untreated controls (CTRL =100%).