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Figure 3 | BMC Complementary and Alternative Medicine

Figure 3

From: Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells

Figure 3

Effects of Equisetum arvense on apoptosis and necrosis induction in primary human lymphocytes. 105 PBMC were cultured for 72 h and left non-stimulated or PHA-L-activated (10 μg/mL) in the presence of medium (CTRL =100%) or in the presence of different Equisetum arvense concentrations (0.05-0.8 μg/mL). Next, the cells were stained with annexin V and propidium iodide and analyzed using flow cytometry. CPT (100 μM) and Triton-X100 (0.5%) were used as apoptosis and necrosis control, respectively. Results from analysis of apoptotic (white bars) and necrotic (black bars) cells are summarized and presented as mean ± SD of four independent experiments and donors. The asterisks represent significant differences from non-treated stimulated cells alone (***P < 0.001).

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