Figure 4

ACE extracts suppressed TNF-α expression with a low cytotoxicity. M1-polarized RAW264.7 was treated graded levels of ACE extracts for 24 h. A. Real-time PCR evaluation of TNF-α mRNA expression in M1-polarized RAW264.7. RAW264.7 without treatment was used as the additional controls. *p < 0.01 and **P < 0.01 versus RAW264.7 cells without treatment, while #p < 0.05 and ##p < 0.01 versus M1-polarized macrophages without treatment. B. The cytotoxicity of ACE extracts was determined by MTS assay, and the results were presented as% of controls. Data are expressed as mean ± SD of three independent experiments done in triplicate.