Figure 5

Effect of HCE-EA on NF-κB activation in LPS-stimulated RAW 264.7 macrophages. Cells were pretreated with HCE-EA (25, 50, 100, or 200 μg/mL) for 2 h prior to incubation with LPS (1 μg/mL) for another 1 h. Protein levels of (A) NF-κB p65 and (B) IκBα and pIκBα were determined by Western blotting analysis. Lamin B was employed as a loading control.