Relative activity of SBE and phosphorylation of Smad3 in NIH/3T3 fibroblasts with different treatments. (A) Relative activity of SBE in the region of type I collagen promoter in murine 3T3 fibroblasts with different treatments. N, non-treated; T, TGF-β1; Y, Yiqihuoxue decoction. The relative activity of SBE in non-treated murine 3T3 fibroblasts was normalized to 1. *, P <0.05; **, P <0.001. (B) Western blotting analysis of p-Smad3 and Smad3 in murine 3T3 fibroblasts with different treatments. p-Smad3, phosphorylated-Smad3. (C) Densitometric analysis of western blotting for p-Smad3 and Smad3. Bars showed the mean ± SD results of three assays. *, P <0.05; **, P <0.001.