Figure 3
Gene expressions and collagen production between SSc dermal fibroblasts with or without Yiqihuoxue treatment. (A) Relative transcript levels of COL1A2, COL3A1, CTGF, SPARC and TGF-β1 in three SSc dermal fibroblasts with or without Yiqihuoxue treatment. N, non-treated; Y, Yiqihuoxue decoction. The expression level of each gene in non-treated group was normalized to 1. *, P <0.05; **, P <0.001. (B) Western blotting analysis of type I collagen (COL1) in the three SSc dermal fibroblasts with or without Yiqihuoxue treatment. (C) Densitometric analysis of western blotting for type I collagen (COL1). Bars showed the mean ± SD results of three assays. *, P <0.05; **, P <0.001.