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Table 1 List of primers, the primer sequences and the primer melting temperature (Tm)

From: Standardized extract of Ficus deltoidea stimulates insulin secretion and blocks hepatic glucose production by regulating the expression of glucose-metabolic genes in streptozitocin-induced diabetic rats

Gene Primer probe Sequence Annealing Tm°C
GK Forward 5′-CATATGTGCTCCGCAGGACTAG-3′ 61.7
Reverse 5′-CTTGTACACGGAGCCATCCA-3′
PEPCK Forward 5′-GCAACTTCTCTCGGCTCGTT-3′ 62.7
Reverse 5′-TGGCAGTTCTACTGGGCTACAC-3′
G6Pase Forward 5′-GGATCTACCTTGCGGCTCACT-3′ 62.7
Reverse 5′-TGTAGATGCCCCGGATGTG-3′
GLUT 2 Forward 5′-CATCAAAACGTAGAGCACGGTAA-3′ 63.4
Reverse 5′-TATGGGCATTTAGTCTGCACGTA-3′
GLUT4 Forward 5′-GCTTGGCTCCCTTCAGTTTG-3′ 63.4
Reverse 5′-CCTACCCAGCCAAGTTGCAT-3′
PPAR γ Forward 5′-CATGAGTTCTTGCGCAGTATCC-3′ 63.4
Reverse 5′-AGAGCATTGAACTTGACAGCAAAC-3′
Β-actin Forward 5′-GCTCTGGCTCCTAGCACCAT-3′ 55
Reverse 5′-GCTGATCCACATCTGCTGGAA-3′
  1. GK: Glucokinase, PEPCK: Phosphoenolpyruvate carboxykinase, G6Pase: Glucose-6-phosphatase, GLUT2: Glucose transporter type 2, GLUT4: Glucose transporter type 4, PPARγ peroxisome proliferator-activated receptors-gamma.