Figure 5

Combined treatments potentiate apoptosis mediated cell death in 1321N1, SW1783 and LN18 cells. (A) Detection of apoptosis using flow cytometry after annexin V-FITC/propidium iodide (PI) staining. 1321N1, SW1783 and LN18 cells were treated with combined treatments at IC50 concentrations for 24 h. Viable cells are in the lower left quadrant (Q3), early apoptotic cells are in the lower right quadrant (Q4), late apoptotic cells are in the upper right quadrant and non-viable necrotic cells are in the upper left quadrant (Q1). (B) Bar graph representing mean values from three independent experiments for i.1321N1, ii. SW1783 and iii. LN18. *P < 0.05 compare to control early or late apoptosis respectively. # P < 0.05 compare to GTT early or late apoptosis respectively. $P < 0.05 compare to HC early or late apoptosis respectively.