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Figure 1 | BMC Complementary and Alternative Medicine

Figure 1

From: Anti-inflammatory and anti-cancer activity of mulberry (Morus alba L.) root bark

Figure 1

Effect of MRBE on NO production (A) and iNOS (B) in LPS-stimulated RAW264.7 cells. RAW264.7 cells were pre-treated with MRBE at the indicated concentrations for 2 h and then co-treated with LPS (1 μg/ml) for the additional 18 h. After treatment, NO production was measured using the media and Griess reagent and cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane, and probed with iNOS antibody for Western blot. iNOS protein was visualized using ECL detection. Actin was used as internal control. DMSO was used as a vehicle. Values given are the mean ± SD (n = 3). *p < 0.05 compared to LPS treatment without MRBE.

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