Table 2 Gene-specific primers used for quantitative real-time PCR of L-6 cells
From: Increasing effect of Tangzhiqing formula on IRS-1-dependent PI3K/AKT signaling in muscle
Gene name | Forward | Reverse |
|---|---|---|
IRS-2 | 5′-AGGCTTGAAGCGGCTAAGTCTCAT-3′ | 5′-TGGCGCTTGGAATTGTGAGCAA-3′ |
AKT | 5′-GTGTGGCAAGATGTGTATGAGAA-3′ | 5′-CAGGCGGTGTGATGGTGAT-3′ |
GluT4 | 5′-GGTTGGTGCCTATGTATGT-3′ | 5′-CGGATGATGTAGAGGTATCG-3′ |
IRS-1 | 5′-AATAGCCGTGGTGATTACAT-3′ | 5′-CAGAAGCAGAAGCAGAGG-3′ |
GAPDH | 5′-AGACAGCCGCATCTTCTTGT-3′ | 5′-TGATGGCAACAATGTCCACT-3′ |