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Table 4 Fold change in gene expression measured by gene array and Q-RT-PCR

From: Berberine moderates glucose metabolism through the GnRH-GLP-1 and MAPK pathways in the intestine

Gene symbol BerL vs DM BerH vs DM
Fold change (gene array) Fold change (Q-RT-PCR) P _value (Q-RT-PCR) Fold change (gene array) Fold change (Q-RT-PCR) P _value (Q-RT-PCR)
Glp1r 3.862 4.6 ± 0.3 0.027 4.231 4.3 ± 0.2 0.026
Gnrh1 −4.615 −3.7 ± 0.2 0.038 −3.758 −3.5 ± 0.3 0.025
Gnrhr −3.715 −3.1 ± 0.4 0.014 −3.271 −2.9 ± 0.4 0.039
Mapk10 6.362 5.9 ± 0.3 0.031 5.837 6.2 ± 0.5 0.015
  1. Glp1r: glucagon-like peptide 1 receptor; Gnrh1: gonadotropin-releasing hormone 1; Gnrhr: gonadotropin releasing hormone receptor; Mapk10: mitogen activated protein kinase 10.
  2. Each Q-RT-PCR assay was repeated using three biological replicates and each analysis consisted of three technical replicates.