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Table 1 Sequences of target gene-specific primers used in real-time PCR

From: Effects of JSOG-6 on protection against bone loss in ovariectomized mice through regulation of osteoblast differentiation and osteoclast formation

Target genes

 

     Sequences

Accession number

ALP

Sense

5′-ATGCCCTGAAACTCCAAA-3′

NM_001287176

Antisense

5′-AGACGCCCATACCATCTC-3′

OPN

Sense

5′-GCTTGGCTTATGGACTGA-3′

NM_001204201

Antisense

5′-GGCAACAGGGATGACATC-3′

OCN

Sense

5′-CAGACAAGTCCCACACAG-3′

NM_007541

Antisense

5′-GCAGAGTGAGCAGAAAGA-3′

OPG

Sense

5′-TGGGAGAAGAACCTTATTTTG-3′

NM_008764

Antisense

5′-CCAGCATCCTCTTTCATAAAG-3′

RANKL

Sense

5′-CCATGAAAACGCAGATTTG-3′

NM_011613

Antisense

5′-CCCTGAAAGGCTTGTTTC-3′

β-actin

Sense

5′-AAGGCCAACCGTGAAAAGAT-3′

NM_007393

Antisense

5′-GTGGTACGACCAGAGGCATAC-3′