Figure 5From: Effects of JSOG-6 on protection against bone loss in ovariectomized mice through regulation of osteoblast differentiation and osteoclast formation Effect of JSOG-6 on the protein levels of OPG, RANKL, and ERK in MC3T3-E1 cells. (A, B) MC3T3-E1 cells (2× 104 cells/mL) were incubated for 48 h and then treated with JSOG-6 for 48 h. After incubation, total cell extracts were obtained and subjected to Western blot analysis as described in the Methods. Data were representative of three separate experiments. β-Actin was used as an internal standard.Back to article page