Effect of JSOG-6 on osteoblastic gene expression. MC3T3-E1 cells (2 × 104 cells/mL) were treated with the indicated concentrations of JSOG-6 for 48 h, and the mRNA levels of osteoblastic genes were examined using real-time PCR. The results are presented as a relative expression level compared to unstimulated cells and were normalized to β-actin. Data represent the mean ± S.D. (n = 3). *P < 0.05, **P < 0.01 indicates statistically significant differences from the control group.