Figure 4

The effect of ethanol extract of Saussurea lappa (ESL) on the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor-kappa B (NF-κB)-related signal proteins in MCF-7 cells. MCF-7 cells were treated with ESL in the presence of TPA. Following 3 h of incubation, nuclear and cytoplasmic extracts were prepared. (B) Western blot analysis was used to determine the cytoplasmic levels of phosphorylated I-kappa B kinase-alpha/beta (p-IKKα/β), I-kappa B kinase-alpha (IKKα), I-kappa B kinase-beta (IKKβ), phosphorylated I-kappa B-alpha (p-IκBα), and I-kappa B-alpha (IκBα); and (A) the nuclear levels of NF-κB (p50 and p65 subunits) and the phosphorylated c-Jun subunit of activator protein-1 (AP-1).