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Figure 2 | BMC Complementary and Alternative Medicine

Figure 2

From: Saussurea lappa extract suppresses TPA-induced cell invasion via inhibition of NF-κB-dependent MMP-9 expression in MCF-7 breast cancer cells

Figure 2

The effect of ethanol extract of Saussurea lappa (ESL) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced metalloproteinase-9 (MMP-9) expression in MCF-7 cells. MCF-7 cell monolayers were treated with the indicated ESL concentrations in the presence of TPA for 24 h. (A) Cell lysates were analyzed by western blot with anti-MMP-9 antibody. The blot was reprobed with anti-β-actin to confirm equal loading. (B) MMP-9 mRNA levels were analyzed by real-time PCR and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. (C) Conditioned medium was prepared and used for gelatin zymography. Each value represents the mean ± SE of three independent experiments. *p < 0.01 vs. TPA.

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