Figure 5

Effect of KZE on the protein levels of MITF and tyrosinase in B16 cells. The cells were treated with 20 μg /ml of KZE for the indicated times. Western blot assays were performed to examine MITF and tyrosinase expression levels. Results were normalized against β-actin expression. Results shown are means ± SEM and are representative of three independent experiments. Data were analyzed by One-Way Analysis of Variance (ANOVA) followed by post hoc Tukey test ^**P < 0.01, compared with control.