Figure 3

MTE ( M. tenacissima extract) enhances gefitinib induced delay in cell cycle in non-small cell lung cancer cell lines H292 (A) and HCC827 (B). Cells were treated with 1 μM gefitinib, 8 mg/ml MTE, and their combination (MTE → MTE + Gef, M → M + G) in 1% FBS culture medium for 72 h, then harvested, ethanol fixed and labeled with PI for the analysis of cell cycle by FACS analysis. Each data presented as the means ± SEM of three experiments. ^* P < 0.05; ^** P < 0.01vs vehicle control group, ^a P < 0.05 vs MTE treated group, ^b P < 0.05 vs gefitinib treated group.