Figure 2

Inhibitory effect of (+)-catechin on oxidative stress-induced cell death in fibroblasts. After incubation with serum-free medium for 24 h, fibroblasts were treated for 30 min with (+)-catechin (0–400 μM) and then subjected to oxidative stress induction with 0.1 mM hydrogen peroxide (H₂O₂). After 24 h, cell viability was evaluated. (a) Representative microscopic images of cell death induced by oxidative stress (a) and inhibitory effect of (+)-catechin on oxidative stress-induced cell death (b). Cell viability was assessed by the MTT assay (c). Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01.