Treatment | Number of PCEs analyzed | PCEMNs | PCE/(PCE + NCE) | NCE ( n) | ||||||
---|---|---|---|---|---|---|---|---|---|---|
24 h | 48 h | 24 h | 48 h | 24 h | 48 h | 24 hA” | 48 hB” | 24 h | 48 h | |
( n)A | ( n)A | (%)A’ | (%)A’ | |||||||
150 mM NaCl | ||||||||||
♀1 | 2095 | 2097 | 7 | 10 | 0.33 | 0.48 | 1.00 | 1.00 | 5 | 3 |
♀2 | 2094 | 2095 | 9 | 10 | 0.43 | 0.48 | 1.00 | 1.00 | 6 | 5 |
♀3 | 2087 | 2089 | 11 | 8 | 0.53 | 0.38 | 0.99 | 0.99 | 13 | 11 |
Σ ♀ | Σ 6276 | Σ 6281 | Σ 27 | Σ 28 | 0.43 ±0.10 | 0.45 ±0.05 | 1.00 ±0.00 | 1.00 ±0.00 | Σ 24 | Σ 19 |
♂1 | 2095 | 2088 | 9 | 13 | 0.43 | 0.62 | 1.00 | 0.99 | 5 | 12 |
♂2 | 2055 | 2088 | 12 | 11 | 0.58 | 0.53 | 0.98 | 0.99 | 45 | 12 |
♂3 | 2058 | 2084 | 7 | 11 | 0.34 | 0.53 | 0.98 | 0.99 | 42 | 16 |
Σ ♂ | Σ 6208 | Σ 6260 | Σ 28 | Σ 35 | 0.45 ±0.12 | 0.56 ±0.06 | 0.99 ±0.01 | 0.99 ± 0.00 | Σ 92 | Σ 40 |
Σ ♂ and ♀ | Σ 12484 | Σ 12541 | Σ 55 A | Σ 63 A | 0.44 ±0.08 A’ | 0.50 ±0.06 A’ | 0.99 ±0.01 A” | 1.00 ±0.00 A” | Σ 116 | Σ 59 |
N–Nitroso–N–ethylurea – NEU (50 mg.Kg –1 ) | ||||||||||
♀1 | 2148 | 2075 | 38 | 36 | 1.77 | 1.73 | 0.49 | 0.65 | 2252 | 1125 |
♀2 | 1884 | 2032 | 32 | 34 | 1.70 | 1.67 | 0.54 | 0.81 | 1616 | 468 |
♀3 | 2002 | 1948 | 15 | 31 | 0.75 | 1.59 | 0.61 | 0.93 | 1298 | 152 |
Σ ♀ | Σ 6034 | Σ 6055 | Σ 85 | Σ 101 | 1.41 ±0.57 | 1.67 ±0.07 | 0.54 ±0.06 | 0.80 ±0.14 | Σ 5166 | Σ 1745 |
♂1 | 2025 | 1999 | 64 | 31 | 3.16 | 1.55 | 0.41 | 0.36 | 2875 | 3501 |
♂2 | 2028 | 1916 | 105 | 40 | 5.18 | 2.09 | 0.51 | 0.55 | 1972 | 1584 |
♂3 | 2004 | 2069 | 25 | 38 | 1.25 | 1.84 | 0.67 | 0.65 | 996 | 1131 |
Σ ♂ | Σ 6057 | Σ 5984 | Σ 194 | Σ 109 | 3.20 ±1.97 | 1.83 ±0.27 | 0.53 ±0.13 | 0.52 ±0.14 | Σ 5843 | Σ 6216 |
Σ ♂ and ♀ | Σ 12091 | Σ 12039 | Σ 279 B | Σ 210 B | 2.30 ±1.66 B’ | 1.75 ±0.18 B’ | 0.54 ±0.06 C” | 0.66 ±0.16 C” | Σ 11009 | Σ 7961 |
Doxorubicin hydrochloride – DXR (5 mg.Kg –1 ) | ||||||||||
♀1 | 2091 | 2017 | 49 | 36 | 2.34 | 1.78 | 0.72 | 0.96 | 809 | 83 |
♀2 | 2106 | 2077 | 73 | 63 | 3.47 | 3.03 | 0.98 | 0.99 | 44 | 23 |
♀3 | 2056 | 2092 | 57 | 50 | 2.77 | 2.39 | 0.84 | 0.95 | 394 | 108 |
Σ ♀ | Σ 6253 | Σ 6186 | Σ 179 | Σ 149 | 2.86 ±0.57 | 2.40 ±0.62 | 0.85 ±0.13 | 0.97 ±0.02 | Σ 1247 | Σ 214 |
♂1 | 2067 | 2086 | 53 | 61 | 2.56 | 2.92 | 0.98 | 0.95 | 33 | 114 |
♂2 | 2063 | 2042 | 56 | 70 | 2.71 | 3.43 | 0.98 | 0.97 | 37 | 58 |
♂3 | 2082 | 2075 | 46 | 50 | 2.21 | 2.41 | 0.99 | 0.99 | 18 | 25 |
Σ ♂ | Σ 6212 | Σ 6203 | Σ 155 | Σ 181 | 2.50 ±0.26 | 2.92 ±0.51 | 0.99 ±0.00 | 0.97 ±0.02 | Σ 88 | Σ 197 |
Σ ♂ and ♀ | Σ 12465 | Σ 12389 | Σ 334 C | 330 C | 2.68 ±0.42 C’ | 2.66 ±0.43 C’ | 0.92 ±0.07 A” | 0.97 ±0.01 A” | Σ 1335 | Σ 411 |
POHALS – Pharmaceutical oil of H. annuus L. seeds (250 mg.Kg –1 ) | ||||||||||
♀1 | 2081 | 2092 | 9 | 7 | 0.43 | 0.33 | 0.99 | 1.00 | 14 | 10 |
♀2 | 2086 | 2087 | 5 | 8 | 0.24 | 0.38 | 0.99 | 1.00 | 13 | 9 |
♀3 | 2090 | 2084 | 8 | 8 | 0.38 | 0.38 | 1.00 | 0.99 | 7 | 12 |
Σ ♀ A A | Σ 6257 | Σ 6263 | Σ 22 | Σ 23 | 0.35 ±0.10 | 0.37 ±0.03 | 0.99 ±0.00 | 1.00 ±0.00 | Σ 34 | Σ 31 |
♂1 | 2082 | 2083 | 10 | 16 | 0.48 | 0.77 | 0.99 | 0.99 | 18 | 11 |
♂2 | 2085 | 2099 | 7 | 9 | 0.34 | 0.43 | 0.99 | 1.00 | 15 | 9 |
♂3 | 2089 | 2072 | 9 | 15 | 0.43 | 0.72 | 0.99 | 0.99 | 11 | 21 |
Σ ♂ B A | Σ 6256 | Σ 6254 | Σ 26 | Σ 40 | 0.42 ±0.07 | 0.64 ±0.18 | 0.99 ±0.00 | 0.99 ±0.00 | Σ 44 | Σ 41 |
Σ ♂ and ♀ | Σ 12513 | Σ 12517 | Σ 48 A | Σ 63 A | 0.38 ±0.09 A’ | 0.50 ±0.19 A’ | 0.99 ±0.00 A” | 0.99 ±0.00 A” | Σ 78 | Σ 72 |
POHALS – Pharmaceutical oil of H. annuus L. seeds (500 mg.Kg –1 ) | ||||||||||
♀1 | 2021 | 2075 | 6 | 6 | 0.30 | 0.29 | 1.00 | 0.99 | 9 | 13 |
♀2 | 2047 | 2087 | 9 | 11 | 0.44 | 0.53 | 0.99 | 1.00 | 11 | 8 |
♀3 | 2034 | 2089 | 8 | 7 | 0.39 | 0.34 | 0.99 | 0.99 | 14 | 11 |
Σ ♀ A A | Σ 6102 | Σ 6251 | Σ 23 | Σ 24 | 0.38 ±0.07 | 0.38 ±0.13 | 0.99 ±0.00 | 0.99 ±0.00 | Σ 34 | Σ 32 |
♂1 | 2055 | 2057 | 10 | 11 | 0.49 | 0.53 | 0.99 | 0.99 | 28 | 18 |
♂2 | 2067 | 2071 | 18 | 15 | 0.87 | 0.72 | 0.99 | 0.99 | 11 | 16 |
♂3 | 2076 | 2082 | 16 | 19 | 0.77 | 0.91 | 1.00 | 0.99 | 7 | 12 |
Σ ♂ B A | Σ 6198 | Σ 6210 | Σ 44 | Σ 45 | 0.71 ±0.20 | 0.72 ±0.19 | 0.99 ±0.01 | 0.99 ±0.00 | Σ 46 | Σ 46 |
Σ ♂ and ♀ | Σ 12300 | Σ 12461 | Σ 67 A | Σ 69 A | 0.54 ±0.24 A’ | 0.55 ±0.24 A’ | 0.99 ±0.00 A” | 0.99 ±0.00 A” | Σ 80 | Σ 78 |
POHALS – Pharmaceutical oil of H. annuus L. seeds (1,000 mg.Kg –1 ) | ||||||||||
♀1 | 2088 | 2091 | 15 | 11 | 0.72 | 0.53 | 0.99 | 1.00 | 12 | 7 |
♀2 | 2084 | 2086 | 9 | 16 | 0.43 | 0.77 | 0.99 | 0.99 | 14 | 14 |
♀3 | 2090 | 2080 | 11 | 9 | 0.53 | 0.43 | 1.00 | 0.99 | 10 | 11 |
Σ ♀ A A | Σ 6262 | Σ 6257 | Σ 35 | Σ 36 | 0.56 ±0.15 | 0.58 ±0.17 | 0.99 ±0.00 | 0.99 ±0.00 | Σ 36 | Σ 32 |
♂1 | 2071 | 2077 | 14 | 23 | 0.68 | 1.11 | 0.99 | 0.99 | 18 | 18 |
♂2 | 2087 | 2093 | 21 | 15 | 1.01 | 0.72 | 0.99 | 0.99 | 13 | 11 |
♂3 | 2084 | 2079 | 17 | 15 | 0.82 | 0.72 | 0.99 | 0.99 | 16 | 17 |
Σ ♂ B A | Σ 6242 | Σ 6249 | Σ 52 | Σ 53 | 0.83 ±0.17 | 0.85 ±0.22 | 0.99 ±0.00 | 0.99 ±0.00 | Σ 47 | Σ 46 |
Σ ♂ and ♀ | Σ 12504 | Σ 12506 | Σ 87 A | Σ 89 A | 0.70 ±0.20 A’ | 0.71 ±0.23 A’ | 0.99 ±0.00 A” | 0.99 ±0.00 A” | Σ 83 | Σ 78 |
POHALS – Pharmaceutical oil of H. annuus L. seeds (1,500 mg.Kg –1 ) | ||||||||||
♀1 | 2091 | 2088 | 15 | 11 | 0.72 | 0.53 | 1.00 | 0.99 | 10 | 12 |
♀2 | 2091 | 2102 | 10 | 15 | 0.48 | 0.71 | 1.00 | 1.00 | 9 | 8 |
♀3 | 2084 | 2076 | 12 | 12 | 0.58 | 0.58 | 0.99 | 0.99 | 19 | 14 |
Σ ♀ A A | Σ 6266 | Σ 6266 | Σ 37 | Σ 38 | 0.59 ±0.12 | 0.61 ±0.10 | 0.99 ±0.00 | 0.99 ±0.00 | Σ 38 | Σ 34 |
♂1 | 2079 | 2084 | 21 | 20 | 1.01 | 0.96 | 0.99 | 0.99 | 21 | 16 |
♂2 | 2083 | 2067 | 18 | 17 | 0.86 | 0.82 | 0.99 | 0.99 | 19 | 21 |
♂3 | 2091 | 2085 | 17 | 21 | 0.81 | 1.01 | 0.99 | 0.99 | 18 | 15 |
Σ ♂ B A | Σ 6253 | Σ 6236 | Σ 56 | Σ 58 | 0.90 ±0.10 | 0.93 ±0.10 | 0.99 ±0.00 | 0.99 ±0.00 | Σ 58 | Σ 52 |
Σ ♂ and ♀ | Σ 12519 | Σ 12502 | Σ 93 A | Σ 96 A | 0.74 ±0.19 A’ | 0.77 ±0.20 A’ | 0.99 ±0.00 A” | 0.99 ±0.00 A” | Σ 96 | Σ 86 |
POHALS – Pharmaceutical oil of H. annuus L. seeds (2,000 mg.kg –1 ) | ||||||||||
♀1 | 2084 | 2091 | 18 | 17 | 0.86 | 0.81 | 0.99 | 1.00 | 17 | 9 |
♀2 | 2089 | 2087 | 16 | 18 | 0.77 | 0.86 | 0.99 | 0.99 | 15 | 13 |
♀3 | 2091 | 2085 | 9 | 13 | 0.43 | 0.62 | 0.99 | 0.99 | 11 | 15 |
Σ ♀ A A | Σ 6264 | Σ 6263 | Σ 43 | Σ 48 | 0.69 ±0.23 | 0.77 ±0.13 | 0.99 ±0.00 | 0.99 ±0.00 | Σ 43 | Σ 37 |
♂1 | 2071 | 2074 | 24 | 25 | 1.16 | 1.21 | 0.98 | 0.98 | 36 | 32 |
♂2 | 2085 | 2086 | 18 | 19 | 0.86 | 0.91 | 0.98 | 0.99 | 33 | 28 |
♂3 | 2048 | 2078 | 15 | 18 | 0.73 | 0.87 | 0.99 | 0.99 | 27 | 30 |
Σ ♂ B A | Σ 6204 | Σ 6238 | Σ 57 | Σ 62 | 0.92 ±0.22 | 0.99 ±0.18 | 0.98 ±0.00 | 0.99 ±0.00 | Σ 96 | Σ 90 |
Σ ♂ and ♀ | Σ 12468 | Σ 12501 | Σ 100 A | Σ 110 A | 0.80 ±0.24 A’ | 0.88 ±0.19 A’ | 0.99 ±0.00 A” | 0.99 ±0.00 A” | Σ 139 | Σ 127 |
POHALS (2 g.kg –1 ) + NEU (50 mg.Kg –1 ) | ||||||||||
♀1 | 2040 | 2054 | 67 | 51 | 3.28 | 2.48 | 0.73 | 0.68 | 760 | 946 |
♀2 | 2042 | 2068 | 61 | 61 | 2.99 | 2.95 | 0.73 | 0.65 | 758 | 1132 |
♀3 | 2039 | 2007 | 54 | 64 | 2.65 | 3.19 | 0.70 | 0.69 | 861 | 893 |
Σ ♀ A A | Σ 6121 | Σ 6129 | Σ 182 | Σ 176 | 2.97 ±0.32 | 2.87 ±0.36 | 0.72 ±0.01 | 0.67 ±0.02 | Σ 2379 | Σ 2971 |
♂1 | 2038 | 2014 | 46 | 52 | 2.26 | 2.58 | 0.64 | 0.69 | 1162 | 886 |
♂2 | 2011 | 2072 | 54 | 59 | 2.69 | 2.85 | 0.67 | 0.80 | 989 | 528 |
♂3 | 2008 | 2053 | 49 | 49 | 2.44 | 2.39 | 0.69 | 0.76 | 892 | 647 |
Σ ♂ B A | Σ 6057 | Σ 6139 | Σ 149 | Σ 160 | 2.46 ±0.21 | 2.61 ±0.23 | 0.67 ±0.03 | 0.75 ±0.05 | Σ 3043 | Σ 2061 |
Σ ♂ and ♀ | Σ 12178 | Σ 12268 | Σ 331 C | Σ 336 C | 2.72 ±0.37 C’ | 2.74 ±0.31 C’ | 0.69 ±0.04 B” | 0.71 ±0.06 B” | Σ 5422 | Σ 5032 |
POHALS (2 g.kg –1 ) + DXR (5 mg.Kg –1 ) | ||||||||||
♀1 | 2034 | 2166 | 76 | 52 | 3.74 | 2.40 | 0.64 | 0.83 | 1166 | 434 |
♀2 | 2069 | 2015 | 51 | 49 | 2.46 | 2.43 | 0.69 | 0.69 | 931 | 885 |
♀3 | 2017 | 2066 | 52 | 59 | 2.58 | 2.86 | 0.70 | 0.69 | 883 | 934 |
Σ ♀ A A | Σ 6120 | Σ 6247 | Σ 179 | Σ 160 | 2.93 ±0.70 | 2.56 ±0.25 | 0.67 ±0.03 | 0.74 ±0.08 | Σ 2980 | Σ 2253 |
♂1 | 2057 | 2017 | 53 | 55 | 2.58 | 2.73 | 0.66 | 0.78 | 1043 | 583 |
♂2 | 2056 | 2037 | 73 | 51 | 3.55 | 2.50 | 0.59 | 0.75 | 1444 | 663 |
♂3 | 2081 | 2021 | 47 | 49 | 2.26 | 2.42 | 0.74 | 0.78 | 719 | 579 |
Σ ♂ B A | Σ 6194 | Σ 6075 | Σ 173 | Σ 155 | 2.80 ±0.67 | 2.55 ±0.16 | 0.66 ±0.08 | 0.77 ±0.01 | Σ 3206 | Σ 1825 |
Σ ♂ and ♀ | Σ 12314 | Σ 12322 | Σ 352 C | Σ 315 C | 2.86 ±0.62 C’ | 2.56 ±0.19 C’ | 0.67 ±0.05 B” | 0.75 ±0.05 B” | Σ 6186 | Σ 4078 |