Figure 5

The effects of B-ext, L-ext, and NAC on Western blot analysis. Western blots showing the effects of B-ext, L-ext, and NAC on blue LED light-induced activation of NF-κB, phosphorylation of p38 MAPK, and conversion of LC3-I to LC3-II in 661 W cells. Cells were pretreated with B-ext, L-ext, or NAC for 1 h, and then exposed to 2500 lx of blue LED light for 3 h. Then, the cells were collected, and lysates were analyzed by western blotting. (A) Representative band images show immunoreactivities against activated NF-κB, NF-κB, phospho-p38, and total p38. (B and C) Quantitative analysis of band densities. (D) Representative band images show immunoreactivities against LC3-I and LC3-II. (E and F) Quantitative analysis of band densities. Data are represented as means ± SEM (n = 5–6). C, control; V, vehicle; B-ext, bilberry extract; L-ext, lingonberry extract. ^## p < 0.01 vs. control; *p < 0.05, **p < 0.01 vs. the vehicle-treated group (Student’s t-test).