Table 2 Validation of microarray data of selected genes by RT-qPCR in Gelsemium s . 2c versus Control 2c treated samples
| Â | Â | Fold change microarray1 | Fold change RT-PCR2 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Symbol | Gene ID | R1 | R2 | R3 | Mean | SEM | R1 | R2 | R3 | Mean | SEM |
AIPL1 | 23746 | -0.60 | -0.59 | -1.06 | -0.75 | 0.13 | n.d. | n.d. | n.d. | Â | Â |
ALPK3 | 57538 | 1.16 | 0.04 | 0.96 | 0.72 | 0.28 | -0.87 | 0.24 | -0.25 | -0.29 | 0.26 |
BIRC8 | 112401 | -0.79 | -0.77 | -0.91 | -0.82 | 0.04 | 1.64 | -1.12 | -1.43 | -0.30 | 0.80 |
C1ORF167 | 284498 | -0.69 | -0.51 | -1.00 | -0.73 | 0.12 | n.d. | n.d. | n.d. | Â | Â |
DDI1 | 414301 | -0.93 | 0.02 | -1.30 | -0.74 | 0.32 | -0.62 | 0.16 | -1.23 | -0.56 | 0.33 |
EN2 | 2020 | -0.62 | -0.13 | -0.77 | -0.51 | 0.16 | -1.53 | -0.12 | -0.41 | -0.69 | 0.35 |
GALR2 | 8811 | -0.57 | -0.36 | -0.72 | -0.55 | 0.08 | -0.94 | -0.19 | -0.61 | -0.58 | 0.18 |
GPR25 | 2848 | -1.15 | 0.20 | -1.08 | -0.68 | 0.36 | -0.74 | 0.60 | -0.11 | -0.08 | 0.32 |
LST1 | 221191 | -0.71 | -0.76 | -1.18 | -0.88 | 0.12 | n.d. | n.d. | n.d. | Â | Â |
OR4X1 | 7940 | -0.96 | -0.55 | -0.74 | -0.75 | 0.10 | n.d. | n.d. | n.d. | Â | Â |
OR5C1 | 390113 | -0.84 | -0.33 | -0.79 | -0.66 | 0.13 | -0.95 | -0.51 | -1.00 | -0.82 | 0.13 |
Klkbl4 | 392391 | -0.05 | -0.70 | -1.04 | -0.60 | 0.24 | -1.10 | -0.17 | -0.41 | -0.56 | 0.23 |
TAC4 | 255061 | -0.23 | -0.28 | -1.34 | -0.62 | 0.30 | -1.14 | -0.10 | -0.46 | -0.57 | 0.25 |