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Table 2 Validation of microarray data of selected genes by RT-qPCR in Gelsemium s . 2c versus Control 2c treated samples

From: Extreme sensitivity of gene expression in human SH-SY5Y neurocytes to ultra-low doses of Gelsemium sempervirens

   Fold change microarray1 Fold change RT-PCR2
Symbol Gene ID R1 R2 R3 Mean SEM R1 R2 R3 Mean SEM
AIPL1 23746 -0.60 -0.59 -1.06 -0.75 0.13 n.d. n.d. n.d.   
ALPK3 57538 1.16 0.04 0.96 0.72 0.28 -0.87 0.24 -0.25 -0.29 0.26
BIRC8 112401 -0.79 -0.77 -0.91 -0.82 0.04 1.64 -1.12 -1.43 -0.30 0.80
C1ORF167 284498 -0.69 -0.51 -1.00 -0.73 0.12 n.d. n.d. n.d.   
DDI1 414301 -0.93 0.02 -1.30 -0.74 0.32 -0.62 0.16 -1.23 -0.56 0.33
EN2 2020 -0.62 -0.13 -0.77 -0.51 0.16 -1.53 -0.12 -0.41 -0.69 0.35
GALR2 8811 -0.57 -0.36 -0.72 -0.55 0.08 -0.94 -0.19 -0.61 -0.58 0.18
GPR25 2848 -1.15 0.20 -1.08 -0.68 0.36 -0.74 0.60 -0.11 -0.08 0.32
LST1 221191 -0.71 -0.76 -1.18 -0.88 0.12 n.d. n.d. n.d.   
OR4X1 7940 -0.96 -0.55 -0.74 -0.75 0.10 n.d. n.d. n.d.   
OR5C1 390113 -0.84 -0.33 -0.79 -0.66 0.13 -0.95 -0.51 -1.00 -0.82 0.13
Klkbl4 392391 -0.05 -0.70 -1.04 -0.60 0.24 -1.10 -0.17 -0.41 -0.56 0.23
TAC4 255061 -0.23 -0.28 -1.34 -0.62 0.30 -1.14 -0.10 -0.46 -0.57 0.25
  1. 1Fold change was calculated as Log2 ratio between G2c and Ct2c expression values within the three replicated experiments (R1-R3); 2fold change was calculated as Log2 transformation of 2-ΔΔCq between G2c and Ct2c. N.d., not detectable.