Figure 1From: Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway in lipopolysaccharide-stimulated RAW 264.7 macrophages Inhibition of NO and PGE2 production by EEPC pre-treatment in LPS-stimulated RAW 264.7 macrophages. Cells were pre-treated with different concentrations of EEPC for 1 h, LPS (0.5 μg/mL) was then added, and cells were incubated for 24 h. The culture supernatants were subject to the nitrite assay (A) and to a PGE2 immunoassay (B). Values represent the mean ± SD of three independent experiments. We assessed differences between mean values by the Student’s t-test. *P < 0.05 indicates significant differences from the LPS-treated group.Back to article page