From: Protective effects of stem bark of Harungana madgascariensis on the red blood cell membrane
Groups | Experiment |
---|---|
A- Experimental groups for the morphological measurements of RBCs | |
1 | RBCs (600 μL) + Extract in Oo 1:1 (21 μL) + WB,Prein 37°C- 30 min, In 37°C- 1 h |
2 | RBCs (600 μL) + CCl4 (6 μL) (in Oo 1:1) + WB, In 37°C- 1 h |
3 | RBCs (600 μL) + CCl4 (6 μL) + WB, In 37°C- 1 h |
4 | RBCs (600 μL) + Extract in Oo 1:1 (21 μL) + WB, Prein 37°C- 30 min, CCl4 (6 μL) (in Oo 1:1), In 37°C - 1 h |
5 | RBCs (600 μL) + WB + PCMB 1 M (60 μL), In 37°C - 1 h |
6 | RBCs (600 μL) + Extract in Oo 1:1 (21 μL) + WB, Prein 37°C - 30 min, PCMB (60 μL) (in Oo 1:1), In 37°C - 1 h |
B- Experimental groups for evaluating the RBCs water permeability in the presence or no of PCMB: NMR measurements | |
1 | RBCs (1200 μL) + WB, Prein 37°C - 30 min, PCMB 1 M (60 μL), In 37°C - 1 h |
2 | RBCs (1200 μL) + extract in Oo, 1:1 (42 μL) + WB, In 37°C - 1 h |
C- Experimental groups for the in vivo study of the extract effects in relation with the lipid peroxidation and the oxidative stress on the RBCs | |
1 | Oo each day for 1 week (1 mL/200 g bw/day) |
2 | Oo each day for 1 week (1 mL/200 g bw/day), CCl4 (2 mL/kg bw/day) in Oo on day 7 |
3 | extract (0.016 g/kg bw/day) 1 week and CCL4 (2 mL/kg bw/day) in the Oo on day 7 |
4 | extract (0.032 g/kg bw/day) 1 week and CCl4 (2 mL/kg bw/day) in the Oo on day 7 |
5 | extract (0.064 g/kg bw /day) 1 week and CCl4 (2 mL/ kg bw/day) in the Oo on day 7 |