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Table 1 Experimental groups for the morphological measurements of RBCs, the RBCs water permeability and the in vivo study of the extract against the oxidative stress

From: Protective effects of stem bark of Harungana madgascariensis on the red blood cell membrane

Groups Experiment
A- Experimental groups for the morphological measurements of RBCs
1 RBCs (600 μL) + Extract in Oo 1:1 (21 μL) + WB,Prein 37°C- 30 min, In 37°C- 1 h
2 RBCs (600 μL) + CCl4 (6 μL) (in Oo 1:1) + WB, In 37°C- 1 h
3 RBCs (600 μL) + CCl4 (6 μL) + WB, In 37°C- 1 h
4 RBCs (600 μL) + Extract in Oo 1:1 (21 μL) + WB, Prein 37°C- 30 min, CCl4 (6 μL) (in Oo 1:1), In 37°C - 1 h
5 RBCs (600 μL) + WB + PCMB 1 M (60 μL), In 37°C - 1 h
6 RBCs (600 μL) + Extract in Oo 1:1 (21 μL) + WB, Prein 37°C - 30 min, PCMB (60 μL) (in Oo 1:1), In 37°C - 1 h
B- Experimental groups for evaluating the RBCs water permeability in the presence or no of PCMB: NMR measurements
1 RBCs (1200 μL) + WB, Prein 37°C - 30 min, PCMB 1 M (60 μL), In 37°C - 1 h
2 RBCs (1200 μL) + extract in Oo, 1:1 (42 μL) + WB, In 37°C - 1 h
C- Experimental groups for the in vivo study of the extract effects in relation with the lipid peroxidation and the oxidative stress on the RBCs
1 Oo each day for 1 week (1 mL/200 g bw/day)
2 Oo each day for 1 week (1 mL/200 g bw/day), CCl4 (2 mL/kg bw/day) in Oo on day 7
3 extract (0.016 g/kg bw/day) 1 week and CCL4 (2 mL/kg bw/day) in the Oo on day 7
4 extract (0.032 g/kg bw/day) 1 week and CCl4 (2 mL/kg bw/day) in the Oo on day 7
5 extract (0.064 g/kg bw /day) 1 week and CCl4 (2 mL/ kg bw/day) in the Oo on day 7
  1. WB = washed buffer, Prein = pre-incubation, In = incubation, Oo: Olive oil, The final volume of 6 mL in each tube was completed with wash buffer. The extract volume (0.064 g of extract / kg bw) prepared with Oo (1:1, m/v - according to previous work) was calculated for a final CCl4 or PCMB tube concentration of 1 mM.