Table 1 Genes up- and down-regulated for at least two of four S. aureus strains following a 30-min exposure to the cranberry product NC90 (2 mg/mL)
ORF SACOL | Gene name | Gene annotation | Fold expressionafor each strain | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
ATCC 29213 | MRSA N315 | SHY97 4023 | SHY97 3906 | DAP b | VAN b | OXA b | OBC c | Vra d | |||
Up-regulated genes | |||||||||||
0263 | Peptidoglycan hydrolase | 15.0 | 24.5 | 3.6 | 9.8 | + | = | = | = | ||
1066 | fmt | Fmt protein | 6.8 | 3.8 | 2.9 | 2.0 | + | + | + | + | + |
1459 | Methionine sulfoxide reductase | 2.3 | 6.0 | −1.5 | 1.2 | = | + | + | + | ||
1490 | pbp2 | Penicillin binding protein 2 | 2.5 | 2.8 | 1.0 | 1.9 | + | + | + | + | + |
1777 | htrA | Putative serine protease Htra | 4.6 | 8.9 | 1.0 | 2.1 | + | + | + | = | |
1932 | Transglycosylase domain protein | 8.5 | 6.8 | 2.2 | 2.5 | + | = | = | + | + | |
1942 | vraR | DNA-binding response regulator | 8.8 | 10.9 | nd | 2.7 | + | + | + | = | + |
1943 | vraS | Sensor histidine kinase VraS | 14.2 | 10.5 | 7.6 | 4.0 | + | + | + | + | + |
1944 | Hypothetical protein | 5.1 | 7.5 | 1.3 | 1.4 | + | + | + | + | + | |
2017 | groES | Chaperonin | nd | 2.2 | 2.3 | 2.2 | + | + | + | = | |
2116 | mur AB | UDP-N-AcGlc-1-carbotransferase | 4.0 | 7.6 | 2.5 | 2.6 | + | + | + | + | + |
2291 | Staphyloxanthin biosynthesis | 6.0 | 2.2 | 4.5 | 3.9 | – | – | – | = | ||
2352 | tcaA | TcaA protein | 5.1 | 6.3 | 1.1 | 1.4 | + | + | + | + | + |
2584 | isaA | Immunodominant antigen A | 2.1 | 1.8 | 2.3 | 2.1 | = | = | = | = | |
Down-regulated genes | |||||||||||
0095 | spa | IgG binding protein | −33.3 | −2.0 | −37.5 | nd | – | = | = | ||
0096 | sarS | Accessory regulator | −2.0 | −2.9 | −2.7 | −2.2 | – | – | – | ||
0136-0150e | capA-O | Capsular polysaccharide | −4.9 | nd | −3.1 | −1.9 | – | = | – | ||
0317 | Lipase precursor | −8.6 | −6.8 | −4.1 | −1.7 | – | – | = | |||
451-452e | ahpFC | Alkyl hydroperoxide reductase | −3.4 | −3.6 | −8.2 | −1.2 | = | = | – | ||
0679 | Na+/H+ antiporter, MnhA | −4.5 | −10.0 | −13.0 | −25.0 | = | = | = | |||
0688-0690e | mntABC | ABC transporter | −43.5 | −66.7 | −86.7 | −91.7 | – | = | = | ||
0765-0766e | saeSR | Sensor/Histidine kinase | −1.5 | −3.7 | −1.5 | −3.6 | = | = | = | ||
1072 | folD | THF dehydrogenase | −2.1 | −2.6 | −2.7 | nd | = | = | = | ||
1079 | purF | amidophosphoribosyltransferase | nd | −10.0 | −0.8 | −2.6 | = | – | – | ||
1140 | isdA | Heme-iron transport | −5.3 | −2.1 | −2.9 | nd | – | = | = | ||
1158-1160e | sdhABC | Succinate dehydrogenase | −5.1 | −7.9 | −4.5 | −3.8 | – | – | = | ||
1173 | hla | Alpha-hemolysin | −3.6 | −4.3 | nd | −2.6 | – | = | = | ||
1262-1263e | sucCD | Succinyl-CoA synthetase | −5.6 | −3.1 | −6.4 | −2.3 | = | = | = | ||
1328 | glnR | Glutamine synthetase repressor | −3.7 | −6.3 | −3.4 | −5.3 | – | – | = | ||
1329 | femC | Glutamine synthetase FemC | −2.7 | −4.2 | −3.2 | −2.9 | – | = | = | ||
1368 | katA | Catalase | −3.2 | −2.4 | −5.2 | −2.1 | = | + | = | ||
1385 | acnA | Aconitate hydratase | −3.4 | −3.0 | −6.3 | nd | = | = | = | ||
1396 | fmtC | FmtC protein | −0.9 | −2.4 | −2.0 | nd | – | – | – | ||
1448-1449e | sucAB | 2-oxoglutarate dehydrogenase | −8.1 | −5.4 | −4.8 | −4.9 | = | = | = | ||
1741 | icd | Isocitrate dehydrogenase, NADP | −5.6 | −2.2 | −11.5 | −2.5 | = | = | + | ||
1742 | gtlA | Citrate synthase | −6.7 | −2.3 | −14.3 | −2.2 | = | = | + | ||
1743 | Amino acid permease | −3.1 | −1.2 | −4.8 | −1.5 | = | = | = | |||
2055 | rsbW | Anti-sigma B factor | −2.6 | nd | −1.6 | −2.1 | – | = | – | ||
2057 | rsbU | Sigma factor B regulator protein | −1.3 | −3.1 | −0.9 | −2.1 | = | = | – | ||
2066-2068e | kdpABC | Potassium-transporting ATPase | −3.5 | nd | −2.3 | −1.7 | = | = | = | ||
2293 | NAD/NADP dehydrogenase | −2.0 | −2.4 | −1.3 | nd | – | = | – | |||
2694 | geh | lipase | −27.3 | −4.3 | −20.0 | nd | = | = | = | ||