Anti-hyperglycemic effects of three medicinal plants in diabetic pregnancy: modulation of T cell proliferation

Table 1 Phytochemical composition of plants

Chemical compounds class	Test		Plant name (part used)
Chemical compounds class	Test		Nauclea latifolia(root and stem)	Picralima nitida(seeds)	Oxytenanthera abyssinica(leaves)
Alkaloids	General test: Dragendorff reagent		+++	-	+++
Alkaloids	Extraction: Mayer reagent		+++	++	+++
Gallic tannins	Saturation of Na acetate + a few drips of FeCl3, 1%		+	+++	++
Cathechic tannins	Stiasny reagent		-	-	+++
Flavonoids	Shinoda reagent (cyanidine reaction)		-	++	++
Anthocyanes	Adding some drips of HCl 5% to 1 mL of decocted + alcalinisation (with drips of ammoniac 50%)		-	+	+
Leucoanthocyanes	Shinoda reagent (chlorhydric alcohol)		-	-	+
Quinonic derivates	Born-Trager reaction	Concentrated HCl	++	+	-
Quinonic derivates	Born-Trager reaction	Diluted HCl	+++	++	-
Saponosides	Foam index (FI) of diluted aqueous decoction (positive if FI ≥ 100, meaning foam height ≥ 1 cm)		+++	-	+
Saponosides			(FI > 1 cm)	(FI < 1 cm)	(FI = 1 cm)
Triterpenoids	Liebermann-Buchard reaction (acetic anhydride-sulfuric acide 50 :1)		-	-	-
Steroids	Kedde reaction (dinitrobenzoic acid 2% in ethanol + NaOH (1 N) 1:1)		-	-	+++
Cardenolids	RAYMOND reaction (Dinitrobenzene 1% in ethanol + NaOH 20%)		-	-	-
Cyanogenic derivates	Grignard reaction (soaked paper with picric acid 5%		-	-	-
Mucilages	Viscosity study (in absolute ethanol)		+++	++	-

Chemical compounds of total brut extract of selected parts of plants. The phytochemical analysis was performed as described in Methods section. (+++) too high, (++) high (+) low: indicates the presence of the compounds in the plants; (−) indicates the absence of compound in plants.

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